| 山绿茶药材质量标准的研究 |
| |
| 引用本文： | 陈勇,谢臻,李立,李兵,李耀华,韦韬. 山绿茶药材质量标准的研究[J]. 中华中医药杂志, 2011, 0(7): 1606-1608 |
| |
| 作者姓名： | 陈勇 谢臻 李立 李兵 李耀华 韦韬 |
| |
| 作者单位： | 广西中医学院药学院;广西南宁食品药品检验所; |
| |
| 基金项目： | 广西科学研究与技术开发计划项目(No.桂科攻032202424); 广西自然科学基金项目(No.桂科自054297)~~ |
| |
| 摘 要： | 目的:建立山绿茶药材的质量标准。方法:采用TLC法鉴别山绿茶药材中芦丁;采用HPLC法测定山绿茶药材中绿原酸与芦丁的含量。结果:TLC鉴别芦丁斑点清晰,绿原酸进样量在0.01082-0.8656μg之间与峰面积呈良好的线性关系,r=1.000,平均回收率为96.53%,RSD为1.41%。芦丁进样量在0.02028-1.6224μg之间与峰面积呈良好的线性关系,r=0.9997,平均回收率为99.15%,RSD为2.00%。结论:所建立的质量控制方法灵敏,简便,重现性好,可用于山绿茶药材的质量控制。
|
| 关 键 词： | 山绿茶 薄层色谱 高效液相色谱 芦丁 绿原酸 |
Study on quality standard of Ilex hainanensis Merr. |
| |
| CHEN Yong,XIE Zhen,LI Li,LI Bing,LI Yao-hua,WEI Tao. Study on quality standard of Ilex hainanensis Merr.[J]. China Journal of Traditional Chinese Medicine and Pharmacy, 2011, 0(7): 1606-1608 |
| |
| Authors: | CHEN Yong XIE Zhen LI Li LI Bing LI Yao-hua WEI Tao |
| |
| Affiliation: | CHEN Yong1,XIE Zhen1,LI Li2,LI Bing1,LI Yao-hua1,WEI Tao1(1Faculty of Pharmacy,Guangxi TCM University,Nanning 530001,China,2Nanning Institute for Food and Drug Control,China) |
| |
| Abstract: | Objective: To establish the method for quality control of Ilex hainanensis Merr..Methods: Rutin of Ilex hainanensis Merr.was identified by TLC.HPLC was used to determine the contents of rutin and chlorogenic acid.Results: Rutin spot on TLC plates was clear.The linear range of chlorogenic acid was good from 0.01082μg-0.8656μg,r=1.000,the average recovery was 96.53%(RSD=1.41%).The linear range of rutin was good from 0.02028μg-1.6224μg,r=0.9997,the average recovery was 99.15%(RSD=2.00%).Conclusion: The method was accurate,simple and reproducible which can be applied to the quality control of Ilex hainanensis Merr.. |
| |
| Keywords: | Ilex hainanensis Merr. TLC HPLC Rutin Chlorogenic acid |
| 本文献已被 CNKI 等数据库收录！ |
|
