An immunocytochemical study of regeneration of gastric epithelia in rat experimental ulcers |
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Authors: | Takanori Matsuoka Michiya Kobayashi Takeki Sugimoto Keijiro Araki |
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Institution: | (1) Department of Tumor Surgery, Kochi Medical School, Oko-cho, Nankoku, Kochi 783-8505, Japan |
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Abstract: | We experimentally observed the process of regeneration of gastric mucosal cells in ulcers induced by surgical removal of the
fundic mucosa of rats. The techniques utilized were immunocytochemistry, laser confocal microscopy, and transmission electron
microscopy (TEM). Routine TEM and PAS reaction were used for parietal cells, chief cells, and mucous cells. As markers of
parietal cells, H+-K+ ATPase, Na+-K+ ATPase, carbonic anhydrase, and aquaporin 4 (AQP4) were used, and for chief cells pepsinogen was used. Healing process of
mucosal defect was as follows. On day 2 after the operation, the single-layered regenerating epithelium (RE) originating from
the marginal epithelium of the ulcer extended over the granulation tissue of the ulcer base towards the center. Regenerating
glands (RGs) appeared in the ulcer margin. The cells appearing first in the RE were undifferentiated cells that had a high
nucleus: cytoplasm ratio and abundant free ribosomes. On day 5, the ulcer was almost filled with RGs. Most cells stained positive
for PAS reaction. A few immature parietal cells stained weakly with H+-K+ ATPase, Na+-K+ ATPase, carbonic anhydrase, and aquaporin-4 antibodies, and a few immature chief cells stained weakly with pepsinogen antibody
were also observed on day 5. On day 10, the ulcer was filled with RGs. The RGs in the periphery of the ulcer stained positive
for markers of mature parietal cells and chief cells, whereas the center of the ulcer was composed of immature parietal cells
and chief cells. By day 25, the mucosal defect was filled with normal gastric glands formed by maturation of the RGs. The
undifferentiated cells that first appeared in the ulcer margin seem to differentiate to special functioning cells of the stomach
5–10 days after ulcer formation. |
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Keywords: | Gastric ulcer Experimental ulcer Epithelial regeneration Immunocytochemistry Parietal cell Chief cell Stomach |
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