施普善对慢性低O2高CO2大鼠空间学习记忆损害的干预作用

目的：观察慢性低O2高CO2大鼠的学习记忆改变及施普善的干预作用。方法：通过八臂迷宫训练筛选的SD大鼠，随机分为对照组（n=8）、慢性低O2高CO2组（n=8）、慢性低O2高CO2＋施普善1mL／kg组（简称施普善1mL／kg组，n=-8）和慢性低O2高CO2＋施普善2．5mL／kg组（简称施普善2．5mL／kg组，n=8）。将慢性低O2高CO2组及施普善干预组（1mL／kg、2．5mL／kg）大鼠放置于低O2高CO2密闭氧舱饲养4周，每天8h，每周6d。施普善干预组每天进氧舱前按既定剂量腹腔注射，慢性低O2高CO2组注射生理盐水。以八臂迷宫和Morris水迷宫实验测定其空间学习记忆功能。结果：与正常对照组相比，慢性低O2高CO2组的工作记忆错误（WME）为（1．31±1．19）次，明显高于正常对照组（0．56±0．50）次（P〈0．05），而施普善1mL／kg组和2．5mL／kg干预组WME均较慢性低O2高CO2模型组明显减少（P〈0．05）。Mortis水迷宫，定位航行测试显示，慢性低O2高CO2模型组第3、第4、第5天的逃避潜伏期均较对照组明显延长（P〈0．05），施普善1mL／kg组与慢性低O2高CO2组相比，第4、第5天的逃避潜伏期明显缩短（P〈0．05），施普善2．5mL／kg组与慢性低O2高CO2组相比，第3、第4、第5天的逃避潜伏期明显缩短（P〈0．05）。空间搜索测试结果显示，慢性低O2高CO2组的平台穿越次数明显少于正常对照组及施普善干预组。结论：慢性低O2高CO2大鼠空间学习记忆受损，短时记忆受损明显，施普善干预可以改善慢性低O2高cO。大鼠模型的空间学习记忆能力。

Intervention of Cerebrolysin on rats'spatial learning memory damage induced by chronic hypoxia-hypercapnia

Objective： To investigate rats＇ spatial learning memory damage induced by chronic hypoxia-hypercapnia and to boserve the protective effects of Cerebrolysin. Methods： Thirty-two of Sprague Dawley rats passed eight-arm radial maze task were intermittently placed in a special closed cabin for 8 h per day and lasting 4 weeks, in which there were O2 9% - 11% and CO2 5%- 6%. Cerebrolysin of 1 ml/kg or 2.5 ml/kg was injected intraperitoneally into the rat separately. Ethology tests were evaluated by eight-arm radial maze task and Morris water maze task. Results： The rats exposured to chronic hypoxia-hypercapnia showed spatial learning memory damage. At eight-arm radial maze task, working memory errors （WME） in chronic hypoxia-hypercapnia group （1.31±1.19） were increased more than that in the control group. Compared with that in chronic hypoxia-hypercapnia group, working memory errors were obviously reduced （P〈0.05） in both Cerebrolysin intervention groups. At Morris water maze task, average escape latency of rat in the chronic hypoxia-hypercapnia group was more longer during the third day to the fifth day than that in the control group （P〈0.05）. Cerebrolysin could more shorten rats＇ average escape latency than that in the chronic hypoxia-hypercapnia group （P〈 0.05）. In the probe test, the numbers crossing the former platform in the chronic hypoxiahypercapnia group were significantly less than those in both control group and cerebrolysin groups.Conclusion： Rats have spatial learning memory disorders, especially remarkable loss of short-term spatial memory, induced by chronical chronic hypoxia-hypercapnia. Cerebrolysin intervention may improve the rats＇ spatial learning memory in chronic hypoxia-hypercapnia model to some extent.