| 线粒体直接PCR扩增法 |
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| 引用本文： | 褚金花,宣登峰,吴萍,张淑华,李国君,耿荣.线粒体直接PCR扩增法[J].首都医学院学报,2004,25(3):284-285. |
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| 作者姓名： | 褚金花 宣登峰 吴萍 张淑华 李国君 耿荣 |
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| 作者单位： | [1]首都医科大学家庭医学与公共卫生学院 [2]丰台区疾病预防控制中心 |
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| 基金项目： | 国家自然科学基金 ( 3 0 0 0 0 14 0 )，教育部科学技术研究重点项目 ( 0 0 13 2 )，北京市科技新星计划 ( 95 5 810 2 80 0 )等资助项目 |
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| 摘 要： | 为建立一种不需提取线粒体DNA而直接用线粒体进行聚合酶链反应 (PCR)的方法 ,将得到的线粒体用高温加热 ,使线粒体膜破裂 ,释放DNA后 ,直接用于PCR扩增。结果 :扩增产物与提纯的线粒体DNA扩增得到的产物一样丰富。提示 :该方法适用于对线粒体DNA纯度没有特别要求的试验
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| 关 键 词： | 线粒体 线粒体DNA 聚合酶链反应 |
| 收稿时间： | 2004-02-16 |
| 修稿时间： | 2004年2月16日 |
Direct Detection of Mitochondria by Polymerase Chain Reaction |
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| Chu Jinhua,Xuan Dengfeng,Wu Ping,Zhan Shuhua,Li Guojun,Geng Rong.Direct Detection of Mitochondria by Polymerase Chain Reaction[J].Journal of Capital University of Medical Sciences,2004,25(3):284-285. |
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| Authors: | Chu Jinhua Xuan Dengfeng Wu Ping Zhan Shuhua Li Guojun Geng Rong |
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| Institution: | 1. College of Public Health and Family Medicine, Capital University of Medical Sciences;2. Fengtai District Center for Disease Control |
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| Abstract: | The aim was to establish a method for polymerase chain reaction (PCR) of mitochondria without mitochondrial DNA extraction. In high temperature, the membrane of mitochondrial was broken and intracellular DNA leaked out. DNA was analyzed by the PCR method. The amplified product was as much as the product which acquired from purified mitochondrial DNA. The studies suggest that this method is adapted to the common mitochondrial DNA amplified test. |
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| Keywords: | mitochondria mitochondrial DNA PCR |
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