应用基因芯片技术对2型糖尿病胰岛素抵抗脂肪信号转导相关基因的研究

目的 应用基因芯片技术比较2型糖尿病胰岛素抵抗(IR)患者与正常人大网膜脂肪组织信号转导相关基因表达谱的差异，以进一步阐明IR的发病机制并探索新的治疗方法。方法 分别以Cy5和Cy3两种荧光染料通过逆转录聚合酶链反应(RT-PCR)将IR组和对照组脂肪组织的mRNA标记成探针，并与载有1组靶基因的基因表达谱芯片进行杂交。通过扫描荧光强度，经计算机软件分析，寻找两组差异表达基因。结果 IR组和对照组之间共筛选出82条差异表达基因。其中信号转导相关差异表达基因19条，表达增加的基因12条，表达降低的基因7条。RT-PCR验证，IR组MKP-4表达明显升高。结论 基因表达谱芯片筛选提示IR的发病涉及多个方面，并与受体信号转导密切相关，为阐明IR的信号转导机制提供强有力的工具。

Study on differentially expressed genes associated with signal transduction in adipose tissues of insulin resistant Type 2 diabetes mellitus by cDNA microarray analysis

Objective To elucidate the mechanism of IR of T2DM and provide a new therapeutic method, the gene expression profiles associated with signal transduction in IR were compared with those of normal omental adipose tissue utilizing cDNA microarray analysis. Methods Both mRNA from IR and the normal omental adipose tissue were reversely transcribed to cDNAs with the incorporation of fluorescent dUTP (cy-5 or cy-3) for preparation of hybridization probes. The mixed probes were hybridized to cDNA microarray, which was scanned for the fluorescent signals and showed differences between the two adipose tissues. Results We identified 82 differently expressed genes between the two tissues, 19 differently expressed genes were associated with signal transduction. In IR 12 genes were upregulated while 7 were downregulated. Conclusion The genes with altered expression and involved in cell proliferation, immune response and metabolism etc, and are correlated with signal transduction. The cDNA microarray provides a powerful approach to elucidate the mechanism of signal transduction of IR.