HSV-1重组蛋白GST-G1为抗原的IgM捕获法ELISA的研究

目的研究单纯疱疹病毒1型(HSV-1)近期感染的IgM酶免疫诊断技术。方法用捕获法ELISA(G-ELISA)和HerpeSelect ELISA(H-ELISA)平行检测1296份孕妇血清HSV-1-IgM。结果 G-ELISA检出阳性血清12份,H-ELISA检出阳性血清11份。G-ELISA多捡出的1份阳性,并经其他试验证实为真阳性。乳胶凝集试验诊断为类风湿因子(RF)阳性的6份血清,经H-ELISA检出阳性血清2份,但经G-ELISA试剂盒检测全为阴性。结论在检测孕妇HSV-1-IgM方面,G-ELISA比H-ELISA可能敏感性更强,特异性更高。G-ELISA是诊断HSV-1近期感染的有效方法。

Research on the in-house capture ELISA for IgM antibody by using HSV-1 recombination Protein GST-G1 as antigen

Objective To develop of an IgM enzyme immunossay for diagnosis of recent HSV - 1 infection.Methods The test used wells of microtiter plates coated with anti - human IgM McAb and sera was added to the wells in the first step.HSV- 1 recombination protein GST-G1 and peroxidase labeled mouse anti-HSV-1 McAb were then incubated.3,3′,5,5′-tetram-ethylbenzidine (TMB) was used as a substrate and the color reaction was read.The in-house capture ELISA(G-ELISA) and Herpe Select ELISA(H-ELISA) were used to measure IgM antibody to HSV - 1 in a total of 1296 serum specimens from pregnant women.Results Twelve IgM -positive serum samples were diagnosed by the G-ELISA and confirmed by the other tests.11/12 IgM - positive serum samples were diagnosed by the H - ELISA.2/6 RF positive sera diagnosed by the late agglutination test,were positive on the H - ELISA but negative on the G-ELISA.Conclusion G-ELISA has higher sensitivity and specificity than H-ELISA in measurement of IgM antibody to HSV- 1 from pregnant women.G-ELISA kit is a tool of diagnosis of recent HSV- 1 infection.