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| 苯妥英钠逆转胶质母细胞瘤多药耐药实验研究 | |
| 引用本文： | 吕华荣,王峻,吴星,戴学元,裴永恩,雷霆. 苯妥英钠逆转胶质母细胞瘤多药耐药实验研究[J]. 实用临床医药杂志, 2011, 15(7): 76-79 |
| 作者姓名： | 吕华荣 王峻 吴星 戴学元 裴永恩 雷霆 |
| 作者单位： | 1. 湖北省黄石市中心医院,神经外科,湖北,黄石,435000 2. 湖北省人民医院,神经外科,湖北,武汉,430060 3. 湖北省武汉同济医院,神经外科,湖北,武汉,430030 |
| 摘 要： | 目的研究不同浓度苯妥英钠(PHT)对化疗耐药胶质母细胞瘤细胞系(8-MG-BA)化疗耐药逆转的影响。方法用不同浓度苯妥英钠对8-MG-BA及H4细胞系处理后,以MTT法检测其对上述两种细胞系化疗耐药逆转的影响,并与维拉帕米(VRP)处理组进行对照。结果用PHT 5、10μg/mL和VRP 5μg/mL处理后,8-MG-BA细胞对卡莫司汀(BCNU)的逆转倍数分别为:25.34、34.97和14.27,对替尼泊苷(VM-26)的逆转倍数分别为:13.44、21.71和11.10。结论 PHT逆转作用大于VRP,且呈现剂量依赖关系。 |
| 关 键 词： | 苯妥英钠 多形性胶质母细胞瘤 多药耐药 逆转 |
Experimental study of phenytoin reverses the multidrug resistance in human glioblastoma cell line |
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| LV Hua-rong,WANG Jun,WU Xing,DAI Xue-yuan,PEI Yong-en,LEI Ting. Experimental study of phenytoin reverses the multidrug resistance in human glioblastoma cell line[J]. Journal of Clinical Medicine in Practice, 2011, 15(7): 76-79 | |
| Authors: | LV Hua-rong WANG Jun WU Xing DAI Xue-yuan PEI Yong-en LEI Ting |
| Affiliation: | 1.Huangshi Central Hospital,Huangshi,Hubei,435000; 2.Hubei Provincial People’s Hospital,Wuhan,Hubei,430060; 3.Tongji Hospital,Wuhan,Hubei,430030) |
| Abstract: | Objective To investigate the reversal of chemotherapy resistance of glioblastoma cell line 8 - MG - BA after incubated in different concentrations of PHT in vitro.Methods After treating with PHT on different concentrations,the reversal of chemotherapy resistance of BCNU and VM - 26 on H4 and 8 - MG - BA cell line was determined by MTT assay.Verapamil treated as control group.Results After treating with PHT 5μg/mL or 10μg/mL,VRP 5μg/mL,the fold reversal of BCNU was 25.34,34.97,14.27 respectively,and that of VM -26 was 13.44,21.71,11.10 respectively. Conclusion Fold reversal was increased as the dose of PHT increased,and fold reversal of PHT was higher than that of VRP. |
| Keywords: | phenytoin glioblastoma multiforme multidrug resistance reverse |
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