珠母贝氨基多糖的分离纯化及其抗肿瘤活性的初步研究

探讨珠母贝氨基多糖的分离纯化及其抗肿瘤活性。马氏珠母贝全脏器经胰酶和枯草杆菌蛋白酶双酶水解、醇沉后得到氨基多糖粗品(CPG)．再经DEAE-52-纤维素柱纯化，并采用MTT法检测其体外抗肿瘤活性。结果表明：CPG和经DEAE-52-纤维素柱纯化级分GAG-1，GAG-2中总氨基己糖的质量分数分别为45．9％，56．5％和59.2％，级分GAG-1硫酸基的质量分数高达13.8％；体外抗肿瘤试验表明CPG，GAG-1和GAG-2具有抑制肿瘤细胞生长的作用，对HL-60细胞的抑制率分别为54．6％。50.0％和35．4％，且能显著增敏化疗药物5-Fu抑制肿瘤细胞的作用，其中GAG-1为主要的活性成分。

Isolation, Purification and its antitumor activity of glycosaminoglycan from Pinctada martensii

Crude glycosaminoglycan(CPG) from the whole viscera of Pinctada martensii was isolated with the two-enzyme hydrolysis and purified by the chromatograph.And the antitumor activity was studied with MTT colormetric assay. The CPG could be separated into two fractions(GAG-1, GAG-2) by the DEAE-52-cellulose ion exchange chromatograph. The contents of hexosamine of CPG,GAG-1, GAG-2 were 54.6%,50.0%,35.4%,respectively. The sulfate of GAG-2 was 13.8%. The inhibiting rates of CPG, GAG-1 and GAG-2 to HL-60 cells were 54.6%, 50.0% and 35.4%,respectively. The inhibition to HL-60 of 5-Fu could be increased by combined use with CPG, GAG-1 and GAG-2, GAG-1 was the main active fraction.