病理性瘢痕中Langerhans细胞的形态学研究

目的：探讨Langerhans细胞与病理性瘢痕的相关性.方法：应用免疫组织化学法（ABC法）观察瘢痕疙瘩、增生性瘢痕及正常皮肤组织中S-100蛋白和CD1a的表达,用透射电镜观察这三种组织中的Langerhans细胞的超微结构.结果：瘢痕疙瘩及增生性瘢痕组织中对S-100蛋白和CD1a免疫反应阳性的Langerhans细胞较正常皮肤中明显增多.瘢痕疙瘩及增生性瘢痕与正常皮肤之间有显著性差异（P＜0.05）.瘢痕疙瘩与增生性瘢痕之间差异不显著（P＞0.05）.电镜下表皮细胞层中Langerhans细胞核形多不规则,胞质中等电子密度,有较多线粒体和溶酶体,扁平囊泡较多.结论：Langerhans细胞在瘢痕疙瘩及增生性瘢痕组织中明显增多,且功能活跃.

Morphological study of Langerhans cells on abnormal scar

Objective To investigate the correlation between the Langerhans cells and abnormal scar. Methods Immunohistochemical technique(ABC method) was performed to observe the expression of S-100 protein and CDla on keolid, hypertrophic scars and normal skin.Using electronmicroscope technique,we study the ultraucture of Langerhans cells in the three kinds of tissues. Results The number of Langerhans cells that the expression of S-100 protein and CDla was positive on keolid or hypertrophic scars is significantly increased comparing with normal skin controls(P<0.05),while there were not significant diffirence between keloid and hypertrophic scars (P>0.05). Under the electronmicroscope,Langerhans cells nucleus mould of the epidermis cell layer is irregular .Cytoplast is the medium electron density with more plastosome, lysosome and flat vesicle. Conclusion The number of Langerhans cells in keolid or hypertrophic scars is significantly increased comparing with normal skin controls,and the function of Langerhans cells in abnormal scar is more active than normal skin,Langerhans cells play an important role in abnormal scar.