光学相干层析成像技术判断大鼠动脉球囊损伤后再狭窄程度

目的探讨光学相干层析成像技术(optical coherence tomography,OCT)判断大鼠动脉球囊损伤后再狭窄程度的可行性。方法将60只雄性SD大鼠随机分成对照组(假手术组30只)和实验组(球囊损伤30只),每个时间点(造模前、造模后2、7、14和28 d)取6只,制备大鼠颈总动脉球囊导管扩张损伤模型,在各个时间点将动脉组织行OCT检测并和血清学指标(LDL、HDL、ALP、CRP)、病理学指标(血管内膜与中膜厚度比值、管腔狭窄率)相比较。结果实验组血清学指标(LDL/HDL、ALP)及病理学指标都随着造模时间逐渐加重,对照组处理前后无明显变化,两组相比差别具有显著意义(P<0.05)。实验组OCT图象散射系数(μs)随造模时间逐渐增大,对照组μs处理前后无明显变化,两组相比差别具有显著意义(P<0.05);实验组μs与病理学指标(血管内膜与中膜厚度比值、管腔狭窄率)均存在高度相关性(P<0.01)。结论 OCT是评估动脉球囊损伤后再狭窄程度的可行方法。

Assessment of Artery Restenosis after Balloon Lesions by Optical Coherence Tomography

Objective To study the feasibility of assessing the extent of artery restenosis after balloon lesions by optical coherence tomography（OCT）.Methods Sixty male SD rats,6 rats at each time point（before modeling and 2,7,14,28 days after modeling） were randomly divided into two groups：control group（sham operation group） and experimental group（ballon injury group）.A balloon injury model of rat thoracic aorta was established.OCT images obtained from the artery were compared with serum biochemistry values and histopathological characteristics.ResultsThe serum biochemistry values and histopathological characteristics showed that the extent of artery restenosis was much more severe in experimental group than in control group（P0.01）.The scattering coefficient（μs） obtained from OCT images in the experimental group kept ascending with the time of model making,which was significantly different from that in the control group（P0.01）.The μs obtained from OCT images showed a high degree of relevance with histopathological characteristics（P0.01）.ConclusionsOCT technique can be used in assessing the extent of artery restenosis after balloon lesions.