8精氨酸加压素_(4-9)肽对衰老脑突触体氨基酸掺入的影响

本文采用分离实触体体外摄取的方法研究8精氨酸加压素_(4-9)(AVP_(4-9))肽片段对青年(3月龄)和衰老(27月龄)大鼠脑的突触体氨基酸掺入和蛋白质合成的影响。结果显示,衰老时海马、额叶和颞叶皮质突触体的~3H—亮氨酸摄取明显减少(P<0.01),AVP_(4-9)肽能够促进上述脑区的突触体的亮氨酸摄取能力,以海马的突触体对AVP_(4-9)肽的反应最强,AVP_(4-9)肽对老年鼠各脑区的促进作用较青年鼠减弱,提示AVP_(4-9)肽对老年性神经精神疾病记忆损害的潜在治疗作用可能与其提高突触体的活性及增加蛋白质合成能力有关。

Effect of Arginine Vasopressin_(4-9) Peptide on the Amino Acid Incorporation in Synaptosome of Aged Rat Brain

Effect of a major metabolite of arginine vasopressin (AVP), AVP_(4-9), on the amino acid incoporation ofsynaptosome was investigated in young (3 months) and old (27 months) rats in vitro. It was shown that therewere decline in H-leucine uptake of synaptosome in the hippocampus, frontal and temporal cortex with aging,and that AVP_(4-9) could enhance H-leucine uptake in the three brain regions of both young and old rats. Increaseof amino acid incorporation induced by AVP_(4-9) was less in old rats than in young rats. The responses of synapto-somes in bippocampus for this peptide were the largest in both young and aged rats. It was suggested that poten-tial therapy action of AVP_(4-9) for memory deficit with aging and geriatric neuropsychiatric diseases might be me-diated by the enhancement of the activity and the protein synthesis of synapsis.