基于网络药理学分析柴胡加龙骨牡蛎汤治疗不寐的作用机制

目的]基于网络药理学技术探讨柴胡加龙骨牡蛎汤治疗不寐的作用机制.方法]采用中药系统药理学分析平台(TCMSP),根据OB≥30％,DL≥0.18以及BATMAN数据库,查找柴胡加龙骨牡蛎汤方中的有效成分,通过Swiss Target Prediction平台进行靶点的预测分析,筛选潜在靶点;通过检索OMIM数据库、...

Analysis of the Effect Mechanism of Modified Chaihu Longgu Oyster Decoction in the Treatment of Insomnia Based on Network Pharmacology

Objective]To study the mechanism of modified Chaihu Longgu Oyster decoction in the treatment of insomnia based on network pharmacology technology.Methods]Through Traditional Chinese Medicine System Pharmacology Analysis Platform(TCMSP) by setting OB≥30%, DL≥0.18 and searching BATMAN database, the active ingredients were found in the modified Chaihu Longgu Oyster decoction, and the potential targets were screened by analysis and prediction through Swiss Target Prediction platform;the insomnia related targets were obtained by searching the databases as OMIM, DiGSeE and GeneCard, and component-target and the other multi-level network association graph were constructed by Cytoscape software. The GO and KEGG enrichment analysis were conducted for the effective targets by DAVID database.Results]A total of 203 active components were screened out by TCMSP and BATMAN, and 161 were obtained after removing the duplicates. According to the OB value and DL value,the top 30 herbal and mineral components were selected for analysis, and a total of 302 potential targets were found. In total of 98 effective targets from the modified Chaihu Longgu Oyster decoction were found after the 2 620 insomnia targets from the disease database intersected with the potential targets in the form of Venny diagram. GO and KEGG enrichment analysis was conducted on the effective targets by DAVID database, and the enrichment results with P<0.05 were selected for analysis according to the p-values from small to large.Conclusion]In this study, it was analyzed for the multi-component, multi-target, multi-pathway of modifed Chaihu Longgu Oyster decoction and its related components-target network effect diagram. It provides the data network support and thus lays a foundation for the later experimental researches in the treatment of insomnia with modifed Chaihu Longgu Oyster decoction.