TT分析法在头颈肿瘤体外药敏试验中的应用

为了选择有效抗癌药提供临床使用以提高化疗疗效 ,用MTT分析 ,对 32例头颈肿瘤术后切下的标本进行体外细胞培养 ,再用 5 -Fu ,VCR ,MTX ,BLM ,DDP ,ADM ,PYM和CTX进行药敏试验 .结果 :对 5 -Fu敏感 2 7例 ,占 84 4% ,VCR敏感 7例 ,占 2 1 9% ;MTX敏感 2 0例 ,占 6 2 5 % ;BLM敏感 2 6例 ,占 81 3% ;DDP敏感 2 6例 ,占 81 3% ;ADM敏感 13例 ,占 40 6 % ;PYM敏感 2 1例 ,占 6 5 6 % ;CTX敏感 1例 ,占 3 1% .32例患者化疗效果满意 ,未出现明显的副作用 ,均存活 3a以上 ,术后常规检查及复查未见复发或转移 .结果表明 :MTT法用于肿瘤细胞药敏试验是一种准确、快速、简便、经济 ,不需放射性同位素 ,结果重复性好的方法 ,值得推广应用 .

Chemosensitivity Test of Human Head and Neck Tumors Cell Line with MTT Assay

In vitro cell culture of head and neck tumors and chemosensitivity test to select sensitivity antitumor bogs for individual chemotherapy. Methods:The chemosensitivity of 32 fresh specimens of human head and neck tumors were tested with MTT assay and eight atitumor drugs. Results:The sensitivity was 84 4% for 5-Fu, 2l 9% for VCR, 62 5% for MTX, 8l 3% for BLM, 8l 3% for DDP, 40 6% for ADM, 65 6% for PYM and 3 l% for CTX. The results of chemotherapy with sensitivity drugs of 32 patients were encouraged. There were no serious side-effects in the chemotherapy and no recurrence and metastasis after the treatment. 32 patients were still alive. Conclusion: Chemosensitivity test of tumor cell lines with MTT assay was accuracy, fast,simple,ecoromical,effective method and was valuable for chemotherapy.