卵巢癌患者术后NDV-ATV-ASI与化疗对免疫功能的影响

目的 :评价新城鸡瘟病毒修饰的自体肿瘤细胞疫苗特异性主动免疫治疗(NDV ATV ASI)及化疗后患者免疫功能的变化 ,为实施肿瘤生物治疗 +化疗联合方案提供依据。方法 :卵巢癌患者术后 3 4例给予NDV ATV ASI及化疗 ,采用流式细胞术免疫荧光双标记法检测患者治疗后外周血单个核细胞 (PBMC)中CD4+ 、CD8+ 细胞内IFN γ和IL 4的表达 ;体外实验以NDV修饰的自体肿瘤细胞与PBMC共培养 (MLTC) ,观察其早期活化分子CD2 5、CD69的表达。结果 :治疗后CD4+ 、CD8+ T淋巴细胞内IFN γ的表达明显增高 ,IL 4表达轻度增高 ,且增高的时间较晚。外周血CD4+ 、CD8+ T淋巴细胞CD2 5、CD69的表达增加。单纯化疗组CD4+ 、CD8+ 细胞内IFN γ表达降低。结论 :NDV ATV ASI可诱导机体产生抗肿瘤免疫应答 ,检测单个T细胞水平的细胞因子可作为一种简单且可靠的免疫治疗效果评价参数 ;而化疗对机体的免疫功能有抑制作用 ,二者联合应用应选择适当的时间。

Analysis of the influence of postoperative NDV-ATV-ASI and chemotherapy on immune status in patients with ovarian cancer

Objective:To evaluate the immune status of patients treated with postoperative NDV ATV ASI and chemotherapy and to provide clinical evidence for the time choice of the combination of chemotherapy and biotherapy.Methods:The alterations of the intracellular cytokines IFN γ and IL 4 at the single cell level during the course of immunization and chemotherapy were determined by using 2 color flow cytometry methods.The expressions of early activation markers CD25 and CD69 were evaluated in CD4 + and CD8 + cells which were stimulated in vitro culturing with NDV modified autologous tumor cells.Results:A remarkable increased level of IFN γ and a delayed increased level of IL 4 were detected in cells from ovarian cancer patients treated with NDV ATV ASI.The expressions of CD69 and CD25 cell surface markers were increased on CD4 + and CD8 + cells after culturing with NDV modified autologous tumor cells.Conclusions:NDV ATV ASI can induce the antitumor immune response in ovarian cancer patients.The cytokines produced in the single T cell level seem to be a simple and reliable parameter for monitoring immunotherapeutical effects.