| 用反义寡核苷酸阻断热休克蛋白70表达诱导卵巢癌细胞凋亡 |
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| 引用本文： | 赵霞,彭芝兰,魏于全.用反义寡核苷酸阻断热休克蛋白70表达诱导卵巢癌细胞凋亡[J].中华医学遗传学杂志,2000,17(1):32-35. |
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| 作者姓名： | 赵霞 彭芝兰 魏于全 |
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| 作者单位： | 1. 610041,成都,华西医科大学附二院妇产科
2. 610041,成都,华西医科大学附一院肿瘤生物治疗中心、肿瘤防治中心 |
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| 基金项目： | 国家杰出青年科研基金项目(39725031)；国家自然科学基金项目(3968003)；四川省卫生厅科研基金项目(962013) |
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| 摘 要： | 目的 观察特异性热体克蛋白７０（ｈｅａｔ－ｓｈｏｃｋ ｐｒｏｔｅｉｎ，ＨＳＰ）反义寡核苷酸阻断卵巢癌细胞的ＨＳＰ７０表达，及其对卵巢癌细胞生长和增殖的影响。方法 用胎盘蓝拒染法计算卵巢癌细胞的生长抑制率。Ｇｉｅｎｓａ染色法从形态上了解凋亡的发生，用琼脂糖凝胶电脉进一步检测发生凋亡的特征性ＤＮＡ降解，流式细胞仪定量分析凋亡发生率及周期性异性。结果 用ＨＳＰ７０反义寡核苷酸处理的卵巢细胞表现有显的生长
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| 关 键 词： | 热休克蛋白70 反义寡核苷酸 卵巢癌 细胞凋亡 |
| 修稿时间： | 1999-03-12 |
Induction of apoptosis in ovarian carcinoma cells by HSP 70 antisense oligodeoxynucleotides |
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| ZHAO Xia,PENG Zilan,WEI Yuquan.Induction of apoptosis in ovarian carcinoma cells by HSP 70 antisense oligodeoxynucleotides[J].Chinese Journal of Medical Genetics,2000,17(1):32-35. |
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| Authors: | ZHAO Xia PENG Zilan WEI Yuquan |
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| Institution: | Department of Obstetrics and Gynecology, Second Affliated Hospital, West China University of Medical Sciences, Chengdu, Sichuan, 610041 P.R. China. yuqua wei@mail.sc.cninfo.net |
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| Abstract: | OBJECTIVE: To investigate the role of HSP70 in the proliferation and survival of ovarian carcinoma cells by inhibiting HSP70 expression with HSP70 antisense oligomer. METHODS: Morphological changes of apoptotic cells were investigated by light microscopy. DNA fragmentation was analysed by agarose gel electrophoresis. Kinetics of induction of apoptosis and cell cycle were analysed by flow cytometry. RESULTS: The HSP70 antisense oligomer treated ovarian carcinoma cells showed apparent inhibition of proliferation and characteristic morphological changes of apoptosis. Also, a ladder-like pattern of DNA fragments was demonstrated on agarose gel electrophoresis. HSP70 antisense-oligomer induced apoptosis of ovarian carcinoma cells mainly in G(1)/S phase in 8.3% to 41% at 1 to 20micromol/L. The apoptosis-inducting effect of HSP70 antisense oligomer was in a dose- and time-dependent manner. CONCLUSION: HSP70 antisense oligomer could not only inhibit the proliferation but also induce the apoptosis in ovarian carcinoma cells. |
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| Keywords: | heat shock protein 70 antisense oligomer carcinoma cells apoptosis |
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