不同浓度乙醇对活体家兔腹腔神经丛毁损的组织学观察

目的以不同浓度乙醇对活体家兔进行腹腔神经丛阻滞毁损,并进行组织病理学观察,为临床治疗提供实验依据。方法成年新西兰大白兔48只,按随机化原则分为手术对照组、50%乙醇组、75%乙醇组和100%乙醇组,各12只。手术对照组给予生理盐水,乙醇各浓度组任选6只共18只在给药1周后活检,其余18只在给药3个月后活检。摘取实验侧腹腔神经节和腹腔神经丛标本,采用Thionine硫堇染色法显示尼氏体,HPIAS-1000病理图文报告系统对尼氏体定量检测平均光密度值。结果 1周后,对照组神经节中细胞清晰可见,核大而圆。50%乙醇组,少量神经元核仁消失,少量髓鞘消失。75%乙醇组和100%乙醇组大部分神经元核仁消失,而胞质内尼氏小体变细,髓鞘大部分消失。3个月后,不同浓度乙醇组神经节镜下观结果与1周相似。图像分析显示,与手术对照组比较,乙醇组尼氏体染色平均光密度明显降低（P〈0.01）;与50%乙醇组比较,75%乙醇组、100%乙醇组1周和3个月尼氏体平均光密度值均明显下降（P〈0.05）;75%乙醇组与100%乙醇组间差异无统计学意义（P＆gt;0.05）。各浓度乙醇组1周与3个月比较,差异无统计学意义（P＆gt;0.05）。结论50%、75%和100%乙醇对兔腹腔神经丛均有不同程度的变性作用,75%乙醇是更为适宜的浓度。

Histological Changes of Neurolytie Celiac-Plexus Block with Various Concentrations of Alcohol in Rabbits

To observe the relationship between histological changes of neurolytie celiac-plexus block and concentrations of alcohol,in order to provide scientific evidence for clinical application. Methods Forty-eight New Zealand rabbits were randomly divided into 4 groups：control group,50% alcohol group,75% alcohol group,and 100% alcohol group （n=12）. Control group was given saline. Eighteen rabbits from alcohol groups （n=6 from each group）were sacrificed one week after administration of alcohol and the rest ones were sacrificed at three month for histological examination. The Nissl body in celiac plexus blocked with alcohol or from the other side without treat-ment were stained with Thionine. HPIAS-1000 system was used to detect OD values of the Nissl body. Results After one week,no abnormality in gangliocytes in tissue sections from control groups was seen;few disappearance of nucleus in neurons was seen in 50% alcohol group;and the majority of nucleus in neurons disappeared in 75% al-cohol group and 100% alcohol group,with smaller Nissl body and demyelination. After three months,the histological changes of alcohol groups were similar to those at one week. The OD values of the Nissl body in alcohol groups were lower than that in control group （P〈0.01）;the OD values of the Nissl body in 75% alcohol group and 100%alcohol group were lower than that in 50% alcohol group at one week and three months （P〈0.05）. No significant difference in OD values of the Nissl body was noted between 75% alcohol group and 100% alcohol group （P〉0.05）. No significant difference was found between one week and three months in each group（P〉0.05）. Conclusion The histological changes of neurolytie celiac-plexus block was related to the concentration of alcohol. 75% alco-hol may be the suitable one for clinical use.