Differential responses of human lens epithelial cells to intraocular lenses in vitro: hydrophobic acrylic versus PMMA or silicone discs |
| |
Authors: | Email author" target="_blank">Qi?YanEmail author Nikole?Perdue E?Helene?Sage |
| |
Institution: | (1) Hope Heart Program, Benaroya Research Institute at Virginia Mason, 1201 Ninth Avenue, 281A, Seattle, WA 98101-2795, USA;(2) Department of Biological Structure, School of Medicine, University of Washington, Seattle, WA 98195, USA;(3) Department of Ophthalmology, School of Medicine, University of Washington, Seattle, WA 98195, USA |
| |
Abstract: | Background The purpose of this study was to determine the influence of different materials of intraocular lenses (IOLs) on human lens
epithelial cell behavior, including adhesion, migration, proliferation, apoptosis, and epithelial-mesenchymal transdifferentiation
(EMT) in vitro.
Methods Human lens epithelial cells (SRA 01/04) were grown on hydrophobic acrylic (Acrysof), polymethylmethacrylate (PMMA), and silicone
IOLs. Cellular adhesion, migration, proliferation, and apoptotic assays were performed to assess cell behavior. The expression
of EMT markers (fibronectin and type I collagen) produced by cells on IOLs was determined by immunoblotting and immunocytochemistry.
Results Human lens epithelial cells exhibited preferred adhesion and reduced apoptosis when cultured on acrylic IOLs, in comparison
to PMMA and silicone IOLs. Cells grown on acrylic lenses formed a confluent epithelial monolayer. Migration of lens epithelial
cells under the acrylic lens was substantially blocked in an in vitro assay. In contrast, cells grown on PMMA and silicone
lenses displayed a spindle-shaped, myofibroblast-like morphology, increased apoptosis, reduced adhesion, and enhanced production
of EMT proteins such as fibronectin and type I collagen. The migration of lens epithelial cells under PMMA and silicone IOLs
was substantial in the in vitro assay.
Conclusion This report demonstrates that hydrophobic acrylic lenses are more capsular biocompatible than PMMA and silicone lenses. The
in vitro assays are reliable measurements for evaluating the responses of human lens epithelial cells to different IOL materials,
and could advance our understanding of the preferential capsular opacification conferred by different IOL materials. |
| |
Keywords: | IOL Lens epithelial cells Epithelial-mesenchymal transdifferentiation Capsular opacification |
本文献已被 SpringerLink 等数据库收录! |
|