中药天年饮对衰老大鼠免疫功能的影响

目的探讨中药天年饮对衰老大鼠免疫功能的影响。方法40只SD大鼠随机分为4组,正常组、衰老模型组、天年饮用药组及阴性对照组,每组均为10只。D-半乳糖连续腹腔注射制作亚急性衰老的大鼠模型。采用ELISA方法检测各组大鼠血清IL-2及IL-6的含量,MTT法检测脾淋巴细胞增殖转化,同时观察巨噬细胞的吞噬功能。结果天年饮可显著提高衰老大鼠血清IL-2含量、脾淋巴细胞的增殖能力以及巨噬细胞的吞噬功能(用药组大鼠与衰老模型组大鼠比较,P<0.01,P<0.05);天年饮可明显降低衰老大鼠血清IL-6的含量(用药组大鼠与衰老模型组大鼠比较P<0.01)。结论中药天年饮可增强衰老大鼠的免疫功能,具有一定延缓衰老的作用。

Influence of Traditional Chinese Medicine Tiannianyin on Immune Function of Aging Rats

Objective To investigate the influence of traditional Chinese medicine Tiannianyin on immune function of aging rats.Methods 40 SD rats were divided into 4 groups at random: normal group,aging model group,Tiannianyin group and negative control group,each group had 10 rats.The subacutely aging model rats were made by injecting D-gal into abdominal cavity continually.ELISA was used to detect the IL-2 and IL-6 contents in serum,MMT to detect the proliferation and transformation of spleen lymphocyte,and the macrophagic phagocytosis was also observed.Results Tiannianyin not only significantly increased the IL-2 content,the proliferation and transformation of spleen lymphocyte and the macrophagic phagocytosis of aging rats(Tiannianyin group compared with aging group: P<0.01,P<0.05);but also obviously decreased the IL-6 content of aging rats(Tiannianyin group compared with aging group: P<0.01).Conclusion Tianninyin can strengthen the immune function of aging rats,so it has fairly anti-aging effects.