微囊藻毒素-LR对小鼠巨噬细胞吞噬功能及活性氧水平的影响

目的观察微囊藻毒素LR(MC-LR)对原代和传代巨噬细胞功能的影响。方法取BALB/c小鼠腹腔巨噬细胞,体外培养液中分别加入终浓度为1,10,100和1000nmol.L-1的MC-LR,同时以巨噬细胞株RAW264.7为对照,分别采用中性红吞噬实验和二氢罗丹明123探针检测实验,测定细胞吞噬活性和细胞内活性氧(ROS)水平。结果MC-LR对小鼠腹腔巨噬细胞的吞噬功能有浓度依赖的抑制作用。当MC-LR浓度高于10nmol.L-1时,小鼠腹腔巨噬细胞的胞内ROS水平也明显降低。但MC-LR对小鼠巨噬细胞系RAW264.7细胞的吞噬功能和细胞内ROS水平均无明显影响。结论MC-LR可抑制小鼠腹腔巨噬细胞的吞噬功能和ROS水平,原代巨噬细胞对MC-LR的敏感性高于传代细胞RAW264.7。

Effect of microcystin-LR on phagocytic function and reactive oxygen species level of mouse macrophages

AIM To investigate the effect of microcystin-LR(MC-LR) on the phagocytic function of mouse peritoneal macrophages and RAW264.7 cells. METHODS Macrophages were isolated from the peritoneal cavity of BALB/c mice and primary culture was performed. The macrophages were incubated with MC-LR 1, 10, 100 and 1000 nmol·L^-1, respectively, for 4 h. Then, the phagocyte ability of peritoneal macrophages to the dye neutral red was measured. The level of reactive oxygen species (ROS) in macrophages was analyzed with dihydrohodamin 123 staining assay. The mouse RAW264.7 cells were treated with MC-LR and the phagocyte ability and ROS level were measured, too. RESULTS MC-LR significantly decreased phagocytic function in a concentration- depended manner, meanwhile the level of ROS in macrophages was notably suppressed when the MC-LR concentration was higher than 10 nmol·L^-1. However, MC-LR didn′t exhibit any obvious effect on mouse RAW264.7 cells. CONCLUSION MC-LR suppresses the phagocytic function and ROS generation of peritoneal macrophages. And the peritoneal macrophages are more sensitive to MC-LR than RAW264.7 cells.