一组抗重组人肝再生增强因子单克隆

目的:制备抗重组人肝再生增强因子(rhALR)单克隆抗体(McAb)。方法:以纯化的rhALR为抗原免疫小鼠,取其脾细胞与SP2/0细胞融合,以ELISA,Westernblot法分别筛选测其滴度及免疫特异性。结果:获得2株稳定分泌抗rhALR单克隆抗体杂交瘤株。其效价,腹水10^-3-10^-5,培养上清10^-2-10^-3,两株McAb针对不同抗原决定簇。结论:制备的抗体具有高特异性。对rhALR在体内组织器官的分布,示踪rhALR在体内的代谢及探索rhALR在翻译水平上对细胞发育的调控分布等研究具有重要的应用价值。

Generation andcharacterization of a series of monoclonal antibodies against recombinant human augmenterof liver regeneration

AIM: To generate monoclonal antibodies against human augmenter of liver regeneration (rhALR). METHODS: After BALB/C mice were immunized by the purified rhALR, the cells of spleen were fused with the cells of SP2/0; The titer and speciality were respectively fathomed from ascites or foster fluid by ELISA and Western-blot test. RESULTS: 2 hybridoma cell lines were successfully obtained. The McAbs titer from ascites and foster fluid are respectively about 10 -3 -10 -5 and 10 -2 -10 -3 . It is evident that the two McAbs were directed at different epitopes. CONCLUSIONS: The McAbs have higher speciality. It is significantly useful of the value that how hALR distribute in tissue organs, how the hALR signals the metabolism in the body and the control distribution of the hALR on cell growth on the translational level and so on is researched.