裸大鼠人高转移肝癌皮下和原位移植模型的建立

目的探讨建立裸大鼠人高转移肝癌的皮下和原位移植模型的可行性，并观察其生物学特性。方法体外培养人高转移肝癌株HCCLM3接种于裸大鼠皮下，建立皮下移植模型，然后用此移植瘤组织再接种于裸大鼠肝内，建立肝原位移植瘤模型。皮下移植的裸大鼠每周称重、测量瘤径，原位移植的裸大鼠每周称重，两组裸大鼠分别于移植8周后处死，通过巨检、镜检等观察移植肿瘤的生物学特性。结果裸大鼠皮下均成瘤，未见肺转移，裸大鼠原位移植成瘤率90％，肺转移70％，腹水发生率50％，自发消退率0％。结论裸大鼠人高转移肝癌HCCLM3移植瘤模型成功率高，原位移植有高转移，无自发消退现象，可作为研究肝癌转移和药物筛选的理想模型。

Establishment of Subcutaneous and Orthotopic Transplant Tumor Model of Hepatocellar Carcinoma Cell Line with High Metastatic Potential in Nude Rat

Objective To study the feasibility of the establishment of subcutaneous and orthotopic transplantation tumor model of hepatocellar carcinoma cell line with highly metastatic potential （HCCLM3） in nude rat, and its tumor biological characteristics. Methods HCCLM3 cell line was inoculated subcutaneously in nude rat to develop growing tumor. Orthotopic implant model was established by suturing histologically intact human tumor tissue on the liver of nude rat. Body weight of nude rat and diameter of tumor were measured weekly. The animals were then killed 8 weeks post transplantation, the biological characteristics of transplant tumor was observed by gross examination and microscopic examination. Results In subcutaneous transplant model, tumor formation rate was 100% and no lung metastasis. In orthotopic transplant model, tumor formation rate was 90%, metastasis rate of lung was 70%, the rate of mass ascites was 50% and the natural extinctive rate was 0%. Conclusion The orthotopic transplantation tumor model in nude rat is an ideal model for studying the metastatic mechanism and screening anti- tumor drugs for liver cancer, in view of its high successful rate and high spontaneous metastatic rate with no natural extinction.