束缚应激致亚健康状态大鼠脑组织Bax、c-Fos的表达及中药干预作用

目的 观察束缚应激致亚健康模型大鼠的脑组织Bax、c-Fos表达情况以及抗衰老片和六味地黄丸的干预作用.方法 取雄性SD大鼠20只,体重320-360g,随机分成4组,即正常对照组、模型对照组、抗衰老片组、六味地黄丸组,每组5只,采用每天限制活动3 h模拟人类束缚应激,建立大鼠亚健康状态模型,造模同时,用抗衰老片、六味地黄丸进行干预.造模14 d结束后,测定大鼠大脑皮质及海马区Bax和c-Fos的表达.结果 造模14 d后,模型对照组大鼠脑组织皮质、海马区Bax的表达面积显著增加（P〈0.05）;给予抗衰老片和六味地黄丸后,大鼠大脑皮质及海马区Bax表达量明显减少（P〈0.05,P〈0.01）;模型对照组大鼠脑组织皮质、海马区c-Fos表达量增加;给予抗衰老片和六味地黄丸后,大鼠的大脑皮质及海马区c-Fos表达有不同程度的减少（P〈0.05,P〈0.01）.结论 束缚应激致亚健康状态的大鼠大脑皮层、海马区Bax、c-Fos表达量增加.抗衰老片和六味地黄丸可减少束缚应激致亚健康状态大鼠脑组织Bax、c-Fos的表达,预防和减少应激引起的神经元损伤,从而改善亚健康状态.

Expression of c-Fos and Bax in Brain Tissue of Sub-health State Rat Induced by Restraint Stress and Interventional Effect of Traditional Chinese Medicine

Objective To observe the expression of Bax and c-Fos in brain tissue of sub-health state rat model induced by restraint stress, and further to study the effect of the Traditional Chinese Medicine. Methods Twenty male SD rats of 320-360g, were randomly divided into 4 with 5 rats per group： normal control group, model control group, Anti-ageing tablets group and Liuwei Dihuang pills group, 5 rats per group. The sub-health state animal model was established by limiting daily movement for 3 hours consecutive to 14 days according to the circumstance that can result human physiological stress. At the same time, the rats in Anti-ageing tablets group and Liuwei Dihuang pills group were treated with Anti-ageing tablets and Liuwei Dihuang pills. Respectively during the model building period.The expression level of Bax and c-Fos in brain tissues were detected on completion of the model establishment. Results Fourteen days after molding, in model control group,the expression level of Bax and c-Fos in cerebral cortex and in hippocampus was increased remarkably, while expression of Bax and c-Fos in anti-aging tablets and Liuwei Dihuang pills treated groups was decreased significantly （P〈0.05, P〈0.01）. Conclusion The rat with mental stress were induced by activity restriction have got the expression of Bax and c-Fos significantly increased in brain tissue . The treatments of anti-aging tablets and Liuweidihuang pills improve this kind of mental stress through reducing the expression of Bax and c-Fos, protecting and decreasing neuron injuries caused by stressors.