微卫星标志在检测白血病抑癌基因缺失中的应用

目的 用微卫星标记技术寻找与白血病发生相关的候选抑癌基因。方法 用聚合酶链反应（PCR）扩增方法检测28例白血病患者11号染色体短臂四个微卫星位点的杂合性缺失频率。结果 18例急性白血病患者中，8例（44．4％）在11号染色体短臂上出现了至少1个位点的杂合性缺失（LOH）；10例慢性白血病患者中未检出所选位点的LOH。其中位点D11S1334（位于11p15.4-p15.2)的LOH率为27．8％（5／18），位点D11S1331（位于11p15.4-p15.1)的LOH率为22．2％（4／18）。位点D11S922（位于11p15.5)LOH率为22．2％（4／18），且有75％（3／4）的患者同时存在该位点与其它位点的改变。结论 在11p15.2-p15.5区域可能存在候选的抑癌基因。

Application of microsatellite markers to the detection of tumor suppressor gene in leukemia

Objective To use microsatellite markers to find and locate the loss of tumor suppressor gene in leukemia. Methods Amplification of 4 microsatellite loci on 11p in 28 cases of leukemia(18 cases of acute leukemia and 10 cases of chronic leukemia) for the loss of heterozygosity(LOH). Results LOH at least one locus was found in 8 of 18 acute leukemia(44.4%), but not in chronic leukemia. Frequency of LOH at locus D11S1334(11p15.4 15.2) and D11S1331(11p15.4 15.1) was 27.8%(5/18) and 22.2%(4/18) respectively. In 4(4/18) cases acute leukemia LOH was observed in D11S922(11p15.5) and in 3 of 4 simultaneous LOH at both this locus and other loci was demon strated. Conclusion Our study indicates that from 11p15.2 to the terminal loci of 11p may harbor a tumor suppressor gene associated with the progression of acute leukemia.