HGF和SAM对慢性肝损伤大鼠肝部分切除术后肝细胞的影响

目的:比较肝细胞生长因子（HGF）和S-腺有蛋氨酸（SAM）对大鼠慢性肝损伤时肝部分切除术后肝再生和肝功能的影响，为临床应用提供依据。方法:以四氯化碳和乙醇联合诱导大鼠慢性肝损伤模型，成模后大鼠行30%肝部分切除术，然后随机分为肝硬化对照组，HGF组，SAM组，HGF+SAM组。手术当天起，肝硬化组肌内注生理盐水2 mL/d，HGF组予腹腔注射肝细胞生长因子0.65 mg/（100g·d），SAM组予肌内注SAM20 mg/（kg·d），HGF+SAM组同时按上述方法和剂量注射2种药物。各组大鼠分别于术后15d被处死，取血检测AST，ALT，ALB和TB； 同时在光镜及电镜下观察肝组织的病理改变及超微结构变化。结果:HGF组，SAM组，HGF+SAM组术后15 d AST，ALT，TB明显低于肝硬化组(P＜0.05)，ALB水平明显高于肝硬化组(P＜0.05)； 而HGF组，SAM组，HGF+SAM组3组之间无统计学差异。结论:肝硬化的大鼠肝脏行部分肝切除术后，肝功能及肝储备功能均有一定程度的受损，肝脏再生亦有障碍。HGF和SAM都可促进术后肝细胞再生。

Effects of HGF and SAM on liver regeneration and liver function in cirrhotic rats after hepatectomy

Abstract:Objective:To comparatively investigate the protective effect of HGF and SAM on liver regeneration and liver function in cirrhotic rats after hepatectomy.Methods :Liver cirrhosis of the rat was successfully induced by injection of CCL4.Then 30% partial hepatectomy was performed in all rats.Cirrhotic rats were divided into 4 groups, namely, cirrhotic group(normal saline 5 mL/d IM, n=16), HGF group(HGF 0.65 mg/(100 g·d) peritoneal injection, n=16), SAM group（SAM 20 mg/(kg·d) IM, n=16）and HGF+SAM group(HGF 0.65 mg/(100g·d), SAM 20 mg/(kg·d), n=16).Animals were sacrificed on the 15th post-operative day to take samples for detection of liver function(AST, ALT, ALB and TB).Liver tissues were also observed under light microscope and electron microscope.Results:In HGF group, SAM group and HGF+SAM group, valus of AST, ALT and TB were decreased significantly as with cirrhotic group(P＜0.05), and concentrations of ALB were increased significantly(P＜0.05).Conclusions:The study suggests that the liver function and regeneration of cirrhotic rats have impaired after partial hepatectomy. However HGF and SAM have the efficacy of enhancing liver regeneration and improving liver function.