| 聚乙二醇化骨形态发生蛋白-2基因转染兔骨髓间充质干细胞及其表达测定 |
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| 引用本文： | 丁立峰,郑刚,杨军,周振东,李建军. 聚乙二醇化骨形态发生蛋白-2基因转染兔骨髓间充质干细胞及其表达测定[J]. 中国骨伤, 2014, 27(1): 48-53 |
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| 作者姓名： | 丁立峰 郑刚 杨军 周振东 李建军 |
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| 作者单位： | 中国医科大学附属盛京医院创伤骨科, 辽宁 沈阳 110004;中国医科大学附属盛京医院创伤骨科, 辽宁 沈阳 110004;中国医科大学附属盛京医院创伤骨科, 辽宁 沈阳 110004;中国医科大学附属盛京医院创伤骨科, 辽宁 沈阳 110004;中国医科大学附属盛京医院创伤骨科, 辽宁 沈阳 110004 |
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| 基金项目： | 辽宁省自然科学基金资助项目（编号：20082118）；辽宁省教育厅科学技术研究资助项目（编号：L2010652） |
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| 摘 要： | 目的：聚乙二醇化骨形态发生蛋白-2基因（PEG/BMP-2）纳米颗粒转染兔骨髓间充质干细胞（rBMSCs），检测骨形态发生蛋白-2（BMP-2）在靶细胞中的表达。方法：原代分离培养rBMSCs，分别采用PEG/BMP-2、脂质体/BMP-2转染细胞，采用流式细胞仪检测转染效率，采用Western Blot和real time RT-PCR方法检测BMP-2表达。结果：成功制备出PEG/BMP-2纳米颗粒并将PEG/BMP-2转染至rBMSCs，转染细胞中BMP-2呈高表达，且与脂质体转染法相比，具有更高的转染效率。结论：PEG/BMP-2纳米颗粒转染rBMSCs可高表达BMP-2，为骨缺损治疗修复提供新的治疗方法。
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| 关 键 词： | 间充质干细胞 骨形态发生蛋白质类 纳米颗粒 转染 兔 |
| 收稿时间： | 2013-06-03 |
Study on bone mesenchymal stem cells transfected by polyethylene glycol/bone morphogenetic protein-2 |
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| DING Li-feng,ZHENG Gang,YANG Jun,ZHOU Zhen-dong and LI Jian-jun. Study on bone mesenchymal stem cells transfected by polyethylene glycol/bone morphogenetic protein-2[J]. China journal of orthopaedics and traumatology, 2014, 27(1): 48-53 |
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| Authors: | DING Li-feng ZHENG Gang YANG Jun ZHOU Zhen-dong LI Jian-jun |
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| Affiliation: | Department of Trauma, Shengjing Hospital of Chinese Medical University, Shenyang 110004, Liaoning, China;Department of Trauma, Shengjing Hospital of Chinese Medical University, Shenyang 110004, Liaoning, China;Department of Trauma, Shengjing Hospital of Chinese Medical University, Shenyang 110004, Liaoning, China;Department of Trauma, Shengjing Hospital of Chinese Medical University, Shenyang 110004, Liaoning, China;Department of Trauma, Shengjing Hospital of Chinese Medical University, Shenyang 110004, Liaoning, China |
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| Abstract: | Objective： Polyethylene glycol/bone morphogenetic protein-2（PEG/BMP-2） nanoparticles were transfected into Rabbit bone mesenchymal stem cells（rBMSCs） and the expression of BMP-2 was detected. Methods： Dissociated rBMSCs were primarily cultured in vitro and BMP-2 gene was transfected into rBMSCs by PEG/BMP-2 nanoparticals and lipofectamine,respectively. The efficiency of transfection was detected by flow cytometry and the expression of BMP-2 was detected by Western Blot and real time RT-PCR. Results： PEG/BMP-2 nanoparticals were successfully synthesized and transfected into rBMSCs. Compared with the lipofectamine transfection group,PEG/BMP-2 transfection group had higher efficiency and higher BMP-2 expression. Conclusion： PEG/BMP-2 nanoparticals transfected rBMSCs highly expressed BMP-2,which provided novel strategies for the treatment of bone defect. |
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| Keywords: | Mesenchymal stem cells Bone morphogenetic proteins Nanoparticals Transfection Rabbits |
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