透皮吸收促进剂对小儿腹泻凝胶膏经皮渗透性的影响

目的： 考察不同透皮促进剂对小儿腹泻凝胶膏体外透皮吸收能力的影响。 方法： 采用药物透皮仪进行小儿腹泻凝胶膏的体外经皮渗透试验，通过单因素试验筛选接受液，并分别考察加入不同质量分数（1%，3%，5%）氮酮、油酸、丙二醇后小儿腹泻凝胶膏剂中吴茱萸碱及吴茱萸次碱的24 h体外累积渗透总量和累积透过率。采用HPLC测定吴茱萸碱及吴茱萸次碱含量，流动相乙腈-水（51：49），检测波长225 nm。 结果： 空白样品中吴茱萸（次）碱在24 h内的累积透过率34.53%，含不同质量分数氮酮样品中吴茱萸（次）碱累积透过率分别为33.92%，34.78%，34.65%，含不同质量分数油酸样品则分别为为33.67%，34.57%，34.57%，含不同质量分数丙二醇样品则分别为34.49%，34.61%，34.53%。 结论： 小儿腹泻凝胶膏剂中不需加入化学透皮吸收促进剂，其所含挥发性成分本身具有促进药物渗透的作用。

Effects of Penetration Enhancers on Percutaneous Penetration of Infantile Diarrhea Gel Ointments

Objective: To investigate effects of different penetration enhancers on in vitro transdermal absorption ability of infantile diarrhea gel ointments. Method: Drug transdermal instrument was adopted to investigate percutaneous penetration of infantile diarrhea gel ointments,acceptable solution was selected by single factor test,and then 24 h in vitro total cumulative permeation and cumulative transmittance of evodiamine and retaecarpine in infantile diarrhea gel ointments were investigated after adding different content(1%,3%,5%) azone,oleic acid,propylene glycol.HPLC was adopted to determine contents of evodiamine and retaecarpine by taking acetonitrile-water(51:49) as mobile phase and detection wavelength of 225 nm. Result: Within 24 h,cumulative transmittance of evodiamine and retaecarpine in blank sample was 34.53%,in samples containing different amounts of azone were 33.92%,34.78% and 34.65%,in samples containing different contents of oleic acid were 33.67%,34.57% and 34.57%,in samples containing different contents of propylene glycol were 34.49%,34.61% and 34.53%,respectively. Conclusion: Volatile components from infantile diarrhea gel ointments had promotion role of drug penetration.