| UPLC-PDA-ESI-MS分析杜仲中化学成分 |
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| 引用本文： | 何峰,王永林,郑林,刘丽君,王爱民,李勇军,黄勇. UPLC-PDA-ESI-MS分析杜仲中化学成分[J]. 中国实验方剂学杂志, 2014, 20(3): 59-62 |
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| 作者姓名： | 何峰 王永林 郑林 刘丽君 王爱民 李勇军 黄勇 |
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| 作者单位： | 贵阳医学院药学院, 民族药与中药开发应用教育部工程研究中心, 贵阳 550004;贵阳医学院药学院, 民族药与中药开发应用教育部工程研究中心, 贵阳 550004;贵阳医学院药学院, 民族药与中药开发应用教育部工程研究中心, 贵阳 550004;贵阳医学院药学院, 民族药与中药开发应用教育部工程研究中心, 贵阳 550004;贵阳医学院药学院, 民族药与中药开发应用教育部工程研究中心, 贵阳 550004;贵阳医学院药学院, 民族药与中药开发应用教育部工程研究中心, 贵阳 550004;贵阳医学院药学院, 民族药与中药开发应用教育部工程研究中心, 贵阳 550004 |
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| 基金项目： | 贵州省科技计划项目（黔科合重大专项字[2012]6009） |
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| 摘 要： | 目的： 建立杜仲药材中化学成分的超高效液相色谱-电喷雾质谱（UPLC-PDA-ESI-MS）分析方法。 方法： 采用ACQUITY UPLC系统，BEH C18色谱柱（2.1 mm×100 mm，1.7 μm），0.1%甲酸乙腈-0.1%甲酸水溶液为流动相，流速0.3 mL·min-1，梯度洗脱，检测波长190~400 nm，柱温45 ℃。Waters电喷雾三重四级杆质谱仪（TQD），负离子模式检测，ESI喷雾电压3 kV，去溶剂气温度350 ℃，去溶剂气（N2）流量650 L·h-1，锥孔气（N2）流量50 L·h-1，扫描范围m/z 100~1 000。 结果： 杜仲中化学成分获得了较好的分离和检测，共鉴定出3个木脂素类、4个环烯醚萜类和4个苯丙素类成分。 结论： 所建方法灵敏度高、分离度好，适用于杜仲药材中化学成分的快速定性鉴定。
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| 关 键 词： | 杜仲 木脂素 环烯醚萜 苯丙素 超高效液相-电喷雾质谱 |
| 收稿时间： | 2013-02-22 |
Identification of Compounds in Herb of Eucommia ulmoides by UPLC-PDA-ESI-MS |
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| HE Feng,WANG Yong-lin,ZHENG Lin,LIU Li-jun,WANG Ai-min,LI Yong-jun and HUANG Yong. Identification of Compounds in Herb of Eucommia ulmoides by UPLC-PDA-ESI-MS[J]. China Journal of Experimental Traditional Medical Formulae, 2014, 20(3): 59-62 |
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| Authors: | HE Feng WANG Yong-lin ZHENG Lin LIU Li-jun WANG Ai-min LI Yong-jun HUANG Yong |
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| Affiliation: | School of Pharmacy, Guiyang Medical College, Engineering Research Center for the Development and Application of Ethnic Medicines and Traditional Chinese Medicine Ministry of Education, Guiyang 550004, China;School of Pharmacy, Guiyang Medical College, Engineering Research Center for the Development and Application of Ethnic Medicines and Traditional Chinese Medicine Ministry of Education, Guiyang 550004, China;School of Pharmacy, Guiyang Medical College, Engineering Research Center for the Development and Application of Ethnic Medicines and Traditional Chinese Medicine Ministry of Education, Guiyang 550004, China;School of Pharmacy, Guiyang Medical College, Engineering Research Center for the Development and Application of Ethnic Medicines and Traditional Chinese Medicine Ministry of Education, Guiyang 550004, China;School of Pharmacy, Guiyang Medical College, Engineering Research Center for the Development and Application of Ethnic Medicines and Traditional Chinese Medicine Ministry of Education, Guiyang 550004, China;School of Pharmacy, Guiyang Medical College, Engineering Research Center for the Development and Application of Ethnic Medicines and Traditional Chinese Medicine Ministry of Education, Guiyang 550004, China;School of Pharmacy, Guiyang Medical College, Engineering Research Center for the Development and Application of Ethnic Medicines and Traditional Chinese Medicine Ministry of Education, Guiyang 550004, China |
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| Abstract: | |
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| Keywords: | Eucommia ulmoides lignans iridoid phenyl propanoid UPLC-PDA-ESI-MS |
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