正交设计优选益心通脉合剂提取工艺

目的：优选益心通脉合剂的水提工艺。方法：以丹参素钠、毛蕊异黄酮葡萄糖苷含量和干膏收率的综合评分为 指标，通过L₉（3⁴）正交试验考察加水量、煎煮时间和煎煮次数对益心通脉合剂水提工艺的影响。采用HPLC测定丹参素钠、毛蕊异黄酮葡萄糖苷含量，检测波长分别为280，260 nm。结果：各因素对水提取工艺的影响顺序为提取次数 >加水量 >煎煮时间，最佳水提工艺为加15倍量水煎煮3次，每次0.5 h；丹参素钠和毛蕊异黄酮葡萄糖苷平均提取量分别为4.928，0.399 5 mg·g^-1。结论：优选的提取工艺稳定可行，适用于益心通脉合剂的规范化生产。

Optimization of Extraction Technology for Yixin Tongmai Mixtures by Orthogonal Test

Objective: To optimize water extraction process of Yixin Tongmai mixtures. Method: With contents of salvianic acid A sodium and calycosin-7-glucoside,yield of dry extract as indexes,L₉(3⁴) orthogonal test was adopted to investigate effects of the amount of water,decocting time and times on extraction technology.HPLC was adopted to determine contents of salvianic acid A sodium and calycosin-7-glucoside with detection wavelength of 280 and 260 nm,respectively. Result: Effects of each factors on water extraction technology was in order of decocting times >the amount of water >extracting time,optimal extraction conditions were as follows:decocted 3 times with 15-fold water,0.5 h each time;Under these conditions,average extracting amounts of salvianic acid A sodium and calycosin-7-glucoside were 4.928 and 0.399 5 mg·g^-1,respectively. Conclusion: Optimized extraction process was stable and feasible,which was suitable for standardization production of Yixin Tongmai mixtures.