天麻-川芎对偏头痛模型大鼠尿液内源性物质代谢的影响

目的： 运用代谢组学方法考察不同配伍比例的天麻-川芎对偏头痛大鼠内源性物质代谢的影响，寻找潜在的生物标志物并分析其代谢途径，为传统中药药对的药理机制研究提供新方法。 方法： 将药材分成4组：天麻组（50 g）、川芎组（50 g）、天麻-川芎A组（天麻-川芎为1：4）、天麻-川芎B组（天麻-川芎为4：1），各组药材分别用10倍量70%乙醇提取。采取注射硝酸甘油的方法建立大鼠偏头痛模型。结合PCA法与PLS-DA法分析不同配伍比例的天麻-川芎对偏头痛大鼠内源性物质影响，结合载荷图信息，寻找生物标志物。 结果： 代谢组学分析表明，与天麻-川芎B组相比，天麻-川芎A组样本点更接近正常对照组，而远离模型组；通过载荷图筛选出16个分类贡献率较大的变量，对其中的9个潜在生物标志物进行了结构鉴定和代谢途径分析。 结论： 代谢组学提供了反映机体整体状态的检测指标，天麻-川芎A组使偏头痛大鼠内源性代谢表型向正常大鼠回归，其作用机制可能涉及到氨基酸代谢及三羧酸循环等代谢过程。

Effect of Gastrodia elata and Ligusticum chuanxiong on Urine Endogenous Metabolism in Migraine Rats

Objective: Using metabonomics method to study the influence of different compatibility proportion of Gastrodia elata and Ligusticum chuanxiong to endogenous metabolites in migraine rats, the purpose of these works was to find potential biomarkers and to analyze the metabolic pathways which can provide new ideas for the pharmacological mechanism of traditional Chinese medicine. Method: The herbs were divided into four groups:G. elata Group, L. chuanxiong group, G. elata and L. chuanxiong A(1:4), G. elata and L. chuanxiong B (4:1). Each group of herbs was extracted with 70% ethanol (10 times the amount of volume). The rat model of migraine was established by injecting nitroglycerin into the organization of rat. The methods of PCA and PLS-DA were used to analysis the influence of different compatibility proportion of G. elata and L. chuanxiong on endogenous metabolites in migraine rat, and search for biomarkers with load map information. Result: The analysis result of metabolomics showed that:compared with G. elata and L. chuanxiong B group, the score points of G. elata and L. chuanxiong A group were more closer to the normal control group, farer from the model group;16 potential biomarkers were selected from the load plot, and 9 potential biomarkers were identified their structure and analyzed metabolic pathways. Conclusion: Metabolomics can provide detection indexes that reflect the metabolic state of the body. G. elata and L. chuanxiong A group can return the endogenous metabolic phenotype of migraine model rat to normal status. Its mechanism may be involved in vivo physiological activity, such as amino acid metabolism and the citric acid cycle.