扎里奴思方对局灶性脑缺血再灌注损伤大鼠血脑屏障通透性的影响

目的： 研究扎里奴思方对局灶性脑缺血再灌注损伤大鼠血脑屏障通透性的影响。 方法： SD大鼠随机分为假手术组、模型组、尼莫地平组、扎里奴思方组；线栓法制备大脑中动脉阻塞缺血再灌注模型；大鼠灌胃给药，于缺血再灌注后1，3，7，14 d取脑，评价神经功能积分，干湿重法测脑含水量，免疫组化法测IgG表达变化。 结果： 与假手术组比较，缺血再灌注后脑含水量、IgG表达显著增高（P < 0.01，P < 0.05），神经功能积分明显降低（P < 0.01）；与模型组比较，尼莫地平组脑含水量、神经功能积分均有不同程度改善（P < 0.05），IgG表达有所下降，尤以3，7 d明显（P < 0.01）；与尼莫地平组比较，扎里奴思方组脑含水量、IgG表达呈不同程度下调（P < 0.05），神经功能积分增大，尤以3，7 d明显；扎里奴思方3，7 d组与1 d比较，IgG含量显著降低（P < 0.05）。 结论： 脑缺血再灌注损伤后1~7 d可引起血脑屏障通透性增加，7 d以后血脑屏障通透性逐渐恢复；扎里奴思方可有效降低缺血再灌注脑含水量及改善脑缺血后神经功能积分，降低IgG在脑内的表达量；其机制可能是通过调控血脑屏障的通透性，抑制有害物质进入脑内，从而发挥脑保护作用。

Effects of Zhali Nusi Fang on Permeability Changes of Blood Brain Barrier after Focal Cerebral Ischemia-reperfusion in Rats

Objective: To research the effects of Zhali Nusi fang(ZLNSF) on the permeability changes of blood brain barrier after focal cerebral ischemia-reperfusion in rats. Method: SD rats were divided randomLy into Sham-operated,model,nimodipine and ZLNSF groups. Rat focal cerebral ischemia-reperfusion model was established by middle cerebral artery occlusion with nylon thread.Afte rats were administered orally with corresponding drugs 1,3,7,14 d,then take out brain.The neurological scores were evaluated.Wet and dry weight measured brain water content;immunohistochemical method to measure IgG expression. Result: Compared with the Sham-operated group, ischemia reperfusion brain water content, IgG expression was significantly increased (P < 0.01, P < 0.05), neurological function score significantly dropped (P < 0.01);compared with the model group, the nimodipine group of brain water content, neurological function score emerged varying degrees of improvement (P < 0.05),the expression of IgG level decreased, especially in 3,7 d significantly (P < 0.01);compared with nimodipine group, ZLNSF group brain water content, IgG expression was emerged varying degrees down (P < 0.05), and nerve function integral increases especially in 3,7 d;compared with 1 d,ZLNSF 3,7 d groups the expression of IgG levels were significantly lower (P < 0.05). Conclusion: Cerebral ischemia-reperfusion injury can induced breakdown and increased permeability of the blood-brain barrier after 1-7 d,then the blood-brain barrier permeability gradually restored after seven days;it is indicated that the treatment of the ZLNSF can promote the cerebral protection. Its mechanism may be related to down-regulate the expression of IgG and reduce the permeability of blood-brain barrier, hold back harmful and large molecules from entering the brain, which plays a critical protective effect.