复方颐神养脑胶囊对多发性脑梗死痴呆大鼠的影响

目的：观察中药复方颐神养脑胶囊（YSYN）对大鼠多发性脑梗死痴呆（MID）的疗效及作用机制。方法：3月龄SD大鼠90只，于左侧颈外动脉注入复合血栓诱导剂，复制多发性脑梗死痴呆模型，动物随机分为模型组、YSYN高剂量组（960 mg·kg^-1）、YSYN中剂量组（480 mg·kg^-1）、YSYN低剂量组（240 mg·kg^-1）、西药阳性组（易倍申）（0.5 mg·kg^-1）以及YSYN合西药阳性药组（480 mg·kg^-1＋0.5 mg·kg^-1），另设10只动物为假手术组，造模后第3天，各组均以10 mL·kg^-1灌胃给药，1次/d，连续4周，模型组和假手术组给予等量蒸馏水。采用Morris水迷宫法进行学习记忆测试；采用酶联免疫法测定海马组织去甲肾上腺素（NE）水平；采用比色法测定海马组织乙酰胆碱酯酶（AchE）、乙酰胆碱转移酶（CHAT）和谷胱甘肽过氧化物酶（GSH-Px）水平。结果：与假手术组比较，模型组大鼠学习记忆能力显著降低（P<0.05，P<0.01），海马组织AchE含量显著升高（P<0.05），海马组织CHAT、NE以及GSH-Px含量显著降低（P<0.01）；与模型组比较，YSYN能够显著提高多发性脑梗死痴呆大鼠学习记忆能力（P<0.05）；显著降低多发性脑梗死痴呆大鼠海马组织AchE含量，提高多发性脑梗死痴呆大鼠海马组织CHAT、NE及GSH-Px含量（P<0.05，P<0.01）。结论：YSYN对MID具有良好的保护作用，其作用机制可能是通过调节海马组织内去甲肾上腺素、乙酰胆碱及谷胱甘肽的活性来实现的。

Influence of Compound Yishen Yangnao Capsule on Multi-infarct Dementia Rats

Objective: To investigate the effect of compound Yishen Yangnao capsule (YSYN) on multi-infarct dementia (MID) rats and its mechanism. Method: The multi-infarct dementia model, were induced by injecting compound thrombosis inducer into the external carotid artery.Sixty SD rats were randomly divided into model group, YSYN high dose group (960 mg·kg^-1),middle dose group (480 mg·kg^-1), low dose group 240 mg·kg^-1, western medicine positive group (Ebixa,0.5 mg·kg^-1), YSYN+Ebixa group (480 mg·kg^-1+ 0.5 mg·kg^-1),sham operation group.From 3 days after modeling, corresponding drugs were given once daily, lasting four weeks. The Morris water maze was used to test the abilities of learning-memory, enzyme-linked immunsorbent was used to assay the levels of norepinephrine(NE) of hippocampal tissue, and chemocolorimetry was used to assay the levels of acetylcholinesterase (AchE), cholinacetyltrans-ferase(CHAT), glutathione peroxidase (GSH-Px). Result: Compared with sham operation group, learning memory abilities of model group rats were significantly decreased (P<0.05,P<0.01), the content of AchE significantly rose (P<0.05) and CHAT and GSH-Px significantly decreased. Compared with model group, the YSYN could significantly improve the abilities of learning memory in MID rats(P<0.05);decreased the level of AchE increased the level of CHAT,NE and GSH-Px (P<0.05,P<0.01). Conclusion: The YSYN could significantly improve the abilities of learning memory in MID rats, and has a good protective effect on MID rats. Its mechanism may adjust the level of NE,AchE,CHAT and GSH-Px of hippocampus tissue.