非小细胞肺癌中EphB4的表达及基因多态性

目的观察EphB4在非小细胞肺癌（NSCLC）中的蛋白表达和基因多态性情况，探讨EphB4与NSCLC的关系。方法采用免疫组织化学染色方法和聚合酶链反应一限制性片段长度多态性（PCR-RFLP）分别检测28例NSCLC患者癌组织和外周血中EphB4蛋白表达和rs314310（C／A）基因多态性情况，并与12例癌旁正常肺组织和30名正常健康人外周血作对比，分析EphB4的蛋白表达和基因多态性与NSCLC的关系。结果NSCLC组织中EphB4的阳性率（53．6％）明显高于癌旁正常肺组织（0．0％），（P〈0．05）；其EphB4表达与分化程度、淋巴结转移和TNM分期有关（P〈0．05或〈0．01），而与性别、年龄和组织来源没有关系（均P〉0．05）。NSCLC患者和正常健康人群外周血中rs314310的CC、CA、AA基因型频率分别为25．O％、53．6％、21．4％和43．3％、53．3％、3．3％，差异均无统计学意义（均P〉0．05）；C和A等位基因频率分别为51．8％、48．2％和70．0％、30．O％，差异均有统计学意义（均P〈0．05）。进一步分析显示，AA基因型患者的EphB4的阳性率明显高于其他基因型患者（P〈0．05）。结论高表达的EphB4在NSCLC的发生发展中有重要的作用，其rs314310AA基因型可能是NSCLC的危险因素。

Genetic polymorphism and protein expression of EphB 4 in non-small cell lung cancer

Objective To investigate the genetic polymorphism distribution and protein expression of EphB 4 in non-small cell lung cancer （ NSCLC ） and to study the possible relationship between EphB 4 and NSCLC. Methods Immunohistochemical method was used to detect the expression level of EphB 4 in 28 tumor tissues. Polymerase chain reaction-restriction fragment length polymorphism PCR （PCR- RFLP） was used for the detection of polymorphism of EphB 4 gene in peripheral blood DNA from 28 NSCLC patients and 30 normal samples. Results EphB 4 gene in cancer tissues was significantly higher than the level of normal organization （53.6% vs O. 0% , P 〈0.05）. The expression level of EphB 4 pro- tein in lung cancer tissues had no significant correlations with the sex, age of the patients, source of the primary cancer（P 〉 0.05 ）, but associated with TNM stages, histological classification, P-TNM status（P 〈 0.05）. The frequencies of rs314310 genotypes CC, CA, AA were 25.0% ,53.6% ,21.4% in NSCLC group and 43.3% ,53.3% ,3.3% in control group respectively. No significant difference was found be- tween the two groups （P 〉 0.05）. The frequencies of EphB 4 C and A allele were 51.8% ,48.2% in con-trol group and 70.0% ,30.0% in lung cancer group respectively. The expression of EphB 4 of AA geno- types was significantly higher than other genotypes （ P 〈 0.05 ）. Conclusion Higher expression of EphB 4 gene associated with the occurrence and development of NSCLC. rs314310 AA genotype might be a risk factor on occurrence of NSCLC.