| PTCH1基因甲基化在胃癌发病中的作用 |
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| 引用本文： | 左云,高军,李兆申,宋宇,涂建成,程志健,王浩,丰宇芳,陈亚楠,刘苏霞,龚燕芳. PTCH1基因甲基化在胃癌发病中的作用[J]. 第二军医大学学报, 2009, 30(8): 884-887. DOI: 10.3724/SP.J.1008.2009.00884 |
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| 作者姓名： | 左云 高军 李兆申 宋宇 涂建成 程志健 王浩 丰宇芳 陈亚楠 刘苏霞 龚燕芳 |
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| 作者单位： | 第二军医大学长海医院消化内科,上海,200433 |
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| 摘 要： | 目的:探讨PTCH1基因甲基化在胃癌发生中的作用及去甲基化试剂5-氮杂-2′-脱氧胞苷(5-Aza-dC)对胃癌的治疗作用。方法:选取10例胃癌组织及其癌旁正常组织和胃癌细胞株AGS,抽提组织和细胞的总RNA和基因组DNA。实时定量QRT-PCR检测PTCH1基因的mRNA表达,MSP(methylation specific PCR)分析PTCH1基因启动子区甲基化变化。用5-Aza-dC处理AGS细胞株,流式细胞术检测细胞周期和凋亡并观察PTCH1基因甲基化水平的改变。结果:胃癌组织、癌旁正常组织和胃癌细胞株AGS中PTCH1基因表达和启动子甲基化水平呈负相关,相关系数为-0.591（P=0.006）;5-Aza-dC的处理可引起胃癌细胞AGS细胞发生凋亡和G0/G1期阻滞,PTCH1基因发生去甲基化变化并表达增加。结论: PTCH1基因启动子区高甲基化是胃癌细胞PTCH1低表达主要原因之一,5-Aza-dC能逆转PTCH1基因甲基化状态,调控其表达,对胃癌具有一定的治疗作用。
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| 关 键 词： | 胃肿瘤；PTCH1基因；Hedgehog信号通路；DNA甲基化； 5-氮杂-2′-脱氧胞苷 |
| 收稿时间： | 2009-01-01 |
| 修稿时间： | 2009-04-20 |
Role of PTCH1 gene hypermethylation in tumorigenesis of gastric cancer |
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| ZUO Yun,GAO Jun,LI Zhao-shen,SONG Yu,TU Jian-cheng,CHENG Zhi-jian,WANG Hao,FENG Yu-fang,CHEN Ya-nan,LIU Su-xi,GONG Yan-fang. Role of PTCH1 gene hypermethylation in tumorigenesis of gastric cancer[J]. Former Academic Journal of Second Military Medical University, 2009, 30(8): 884-887. DOI: 10.3724/SP.J.1008.2009.00884 |
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| Authors: | ZUO Yun GAO Jun LI Zhao-shen SONG Yu TU Jian-cheng CHENG Zhi-jian WANG Hao FENG Yu-fang CHEN Ya-nan LIU Su-xi GONG Yan-fang |
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| Affiliation: | Department of Gastroenterology,Changhai Hospital,Second Military Medical University,Shanghai 200433,China |
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| Abstract: | Objective:To study the effect of PTCH1 methylation on gastric carcinogenesis and the therapeutic effect of methylation inhibitor,5-aza-2'-deoxyeytidine(5-aza-dC),for treatment of gastric cancer.Methods:The total RNAs were extracted from 10 gastric cancer tissues,their corresponding adjacent normal tissues,and gastric cancer cell line AGS.The PTCH1 mRNA expression was detected by Quantitative real-time PCR(QRT-PCR) and the methylation of the promoter was examined by methylation specific PCR(MSP).AGS cells we... |
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| Keywords: | stomach neoplasms PTCH1 gene hedgehog signal pathway methylation 5-aza-2'-deoxyeytidine |
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