钴铬合金表面改性对4种微生物黏附的影响

目的研究氮离子溅射和氮等离子浸没注入对钴铬合金表面微生物黏附附能力的影响。方法制作钴铬合金试件288件，各随机选出96件，分别采用氮离子溅射和氮等离子浸没注入对其表面改性。钴铬合金组为对照组，氮离子溅射组为实验组1，氮等离子浸没注入组为实验组2。在实验组1、2组和对照组试件表面黏附血型链球菌、黏性放线菌、白色念珠菌和金黄色葡萄球菌，分别进行微生物体外黏附实验。用菌落形成单位计数法统计分析氮离子溅射和氮等离子浸没注入对各种微生物黏附量的影响。结果在微生物黏附24h、48h和168h后．上述4种微生物在氮离子溅射组和氮等离子浸没注入组表面黏附量较对照组表面黏附量显著减少（P〈0．001），其中4种微生物在氮等离子浸没注入组表面黏附量明显少于氮离子溅射组表面（P〈0．001）。结论钴铬合金表面氮离子溅射和氮等离子浸没注入均可抑制微生物黏附，氮等离子浸没注入较氮离子溅射抑制微生物黏附效果更明显。

Impact of surface modification on Co-Cr alloy on adhesion of microorganisms

Objective To study the effect of N^＋ ion sputtering and N^＋ plasma ion implantation on the microorganisms adhesion to the surface of Co-Cr alloy. Methods Two hundred and eighty-eight pieces of Co-Cr alloy were made, of them ninety-six pieces were randomly chosen, coated with TiN on surface by N^＋ ion sputtering and N^＋ plasma ion implantation, and designated as experimental group 1 and group 2, respectively, while those of non-coated ones as the control group. Definite amounts of Streptomyces sanguis, Actinomyces viscosus, Candida albicans and Staphylococcus aureus were adhered onto the surface of Co-Cr alloy, and then the number of microorganisms was assayed by clone forming unit （CFU） method. Results At 24 h, 48 h and 168 h time points, the number of the adhered microorganisms was significantly reduced in both group 1 and group 2 （P 〈 0.001 ）, but the effect was more significant in group 2 than group 1 （ P 〈 0.001 ）. Conclusion Both N^＋ ion sputtering and N^＋ plasma ion implantation on the surface of commonlyused clinical Co-Cr alloy can reduce adhesion of microorganisms, and N^＋ plasma ion implantation method exhibit more significant effect on reducing adhesion of microorganisms on Co-Cr alloy than the N^＋ ion sputtering method.