Mechanisms affecting neutrophil migration capacity in breast cancer patients before and after chemotherapy |
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Authors: | Maria Angélica Oliveira Mendonça Fabrício O Souto Douglas C Micheli José Carlos Alves-Filho Fernando Q Cunha Eddie Fernando C Murta Beatriz M Tavares-Murta |
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Institution: | 1. Institute of Natural and Biological Sciences, Federal University of Triangulo Mineiro (UFTM), Uberaba, Minas Gerais, Brazil 4. Faculty of Medicine, Federal University of Uberlandia, Avenida Pará, 1720, Bloco 2U, Uberlandia, MG, 38400-902, Brazil 2. Department of Pharmacology, Faculty of Medicine of Ribeir?o Preto, University of S?o Paulo, Ribeir?o Preto, S?o Paulo, Brazil 3. Oncological Research Institute (IPON), Discipline of Gynecology and Obstetrics, Federal University of Triangulo Mineiro (UFTM), Uberaba, Brazil
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Abstract: | Purpose To investigate the mechanisms affecting neutrophil migration capacity in breast cancer patients before and after chemotherapy. Methods Peripheral venous blood was collected at the time of diagnosis and immediately prior to the 4th cycle of an anthracycline-based chemotherapy regimen for patients diagnosed with different stages of breast cancer (n = 30), for experimental assays. Blood samples were also collected from a healthy control group (n = 17). Results IL-8 serum concentrations were higher in the patient group than in the control group (p = 0.02), and chemotherapy did not further affect this increase. Levels of TNF-α, IL-6, and IL-10 did not differ between controls and patients, or in relation to chemotherapy. Serum levels of nitric oxide (NO) metabolites were elevated following chemotherapy compared to levels detected prior to treatment (p = 0.01). When the supernatants of lipopolysaccharide-stimulated mononuclear cells and neutrophils obtained from the patients were assayed for levels of nitrite, these levels were significantly higher and unchanged, respectively, compared with controls. Expression levels of the chemokine receptors, CXCR1 and CXCR2, were significantly reduced in patients compared to controls, and chemotherapy did not further affect these differences. Furthermore, filamentous actin content for IL-8-activated neutrophils was reduced with chemotherapy (median 8.85; range 3.38–13.43) compared to the content detected prior to treatment (median 9.23; range 2.86–22.16) (p = 0.001). Conclusion Elevated systemic levels of IL-8 and NO, desensitization to CXCR activation, and reduction in actin polymerization may affect neutrophil motility in patients before and after chemotherapy. |
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