| HPLC测定盐酸土霉素的含量和有关物质 |
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| 引用本文： | 陈宁林,沈佳特,张春瑛. HPLC测定盐酸土霉素的含量和有关物质[J]. 华西药学杂志, 2001, 16(1): 56-57 |
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| 作者姓名： | 陈宁林 沈佳特 张春瑛 |
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| 作者单位： | 湖北省药品检验所, |
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| 摘 要： | 目的 :建立HPLC测定盐酸土霉素有关物质和含量。方法 :采用ODS柱 ( 10 μm ,4 6mm× 2 5 0mm) ,以 0 1mo1·L-1草酸铵溶液 二甲基甲酰胺 0 2mo1·L-1磷酸氢二铵溶液 ( 6 0∶30∶10 ) (用氨试液调pH8 3)为流动相 ,检测波长 2 70nm。结果 :盐酸土霉素在 0 1～ 1 0mg·ml-1浓度范围内 ,呈良好的线性关系 (r =0 9999) ,精密度RSD为1 2 % ,平均回收率为 99 6 % ,RSD为 0 8% (n =4)。结论 :该法简便、准确 ,适用于盐酸土霉素杂质的检测和含量的测定。
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| 关 键 词： | 高效液相色谱 盐酸土霉素 有关物质 含量测定 |
| 文章编号： | 1006-0103（2001）-01-0056-02 |
| 修稿时间： | 2000-06-01 |
DETERMINATION OF OXYTETRACYCLINE HYDROCHLORIDE AND RELATED SUBSTANCES BY HPLC |
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| CHEN Ning-lin,SHENG Jia-te,ZHANG Chun-ying. DETERMINATION OF OXYTETRACYCLINE HYDROCHLORIDE AND RELATED SUBSTANCES BY HPLC[J]. West China Journal of Pharmaceutical Sciences, 2001, 16(1): 56-57 |
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| Authors: | CHEN Ning-lin SHENG Jia-te ZHANG Chun-ying |
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| Abstract: | OBJECTIVE:An HPLC method was established to determine oxytetracycline hydrochloride and related substances.METHODS:The liquid chromatographies equipped with 270 nm detector,μ Bondapak ODS column(4.6 mm×250 mm, 10 μm). The mobile phase is 0.1 mol*L-1 ammonium oxalate-dimethylformamide-0.2 mol*L-1 dibasic ammoniumphosphate(60 ∶30∶10)（adjusted with 3 mol*L-1 ammonium hydroxide to pH 8.3）.RESULTS:The linearity is good (r=0.9999) in the range of 0.1～1.0 mg*ml-1.The recovery rate was 99.6%, RSD＝0.8%.CONCLUSION:The method is simple,accurate and is very suitable for determination of oxytetracycline hydrochloride and related substances. |
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| Keywords: | HPLC Oxytetracycline hydrochloride Related substances |
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