氧自由基对肾性贫血大鼠红细胞膜脂质过氧化物和Na^＋-K^＋ATP酶的影响

目的：采用鼠婴肾组织肾包膜下移植的方法治疗大鼠的肾性贫血,进一步研究氧自由基对肾性贫血大鼠红细胞膜脂质过氧化物和Na^＋-K^＋ATP酶的影响。方法：以Wistar雄性大鼠建立慢性肾衰竭动物模型为受体,将鼠婴肾组织块多点植入受体肾包膜下。治疗期间监测血红蛋白（Hb）、红细胞（RBC）和血清肌酐（Scr）、血清尿素氮（BUN）,红细胞膜脂质过氧化物（MDA）、超氧化物歧化酶（SOD）、Na^＋-K^＋ATP酶的活性。结果：60d时肾组织移植治疗组Hb含量（106±12）g/L高于模型对照组（80±5）g/L,P〈0.05;RBC数（6.05±0.60）×10^12/L高于模型对照组（5.15±0.23）×10^12/L,P〈0.05;红细胞SOD含量（1557±54）U/gHb增加,与模型对照组（1051±36）U/gHb比较,P〈0.05;红细胞膜Na^＋-K^＋ATP酶水平（178.5±14.5）μmolp/gHb增加与模型对照组（88.7±20.1）μmolp/gHb比较,P〈0.05;红细胞MDA含量（1.87±0.25）nmol/ml低于模型对照组（3.73±0.36）nmol/ml,P〈0.05。结论：同种肾组织移植后氧自由基对红细胞膜的损伤程度减轻,膜的流动性增加,膜对Na^＋离子转运及能量代谢得到适当调整,稳定了红细胞膜的结构和功能。

The Effects of RBC-SOD to RBC-MDA and Na+-K+ATPase in Renal Anemia Rats Caused by Chronic Renal Failure

Objective： To study effction of RBC - SOD to RBC - MDA and Na^＋ - K^＋ ATPase in renal anemia rats caused by chronic renal failure , kidney tissue mass implantation into the capsule of rat kidney to treat anemia caused by chronic renal failure （CRF）. Methods：After the models of chronic renal failue （CRF） induced by adriamyucin were set up in Wistar rats,the kidney tissue mass （ 1 mm3） of newborn Wistar rats were located under the capsule of kidney of the models. During the process of treatment, Hb, RBC, BUN, Scr in blood were tested by automatic analyzer, RBC- SOD, RBC- MDA , Na^＋ -K^＋ ATPase level were tested. Results： the levels of I/b（106 ± 12） g/L, RBC（6.05 ± 0. 60） × 10^12/L in blood kept on increasing in the period of implantation and were higher than Hb（80 ± 5 ） g/L, RBC（5.15 ± 0.23） × 10^12/L, RBC - SOD（ 1 557 ± 54） U/g Hb, RBC - MDA（ 1.87 ± 0.25 ） nmol/ml, Na^ ＋ -K^ ＋ ATPase leve（ 178.5 ± 14.5）μmolp/g Hb kept on increasing in the period of implantation and were higher than RBC - SOD（ 1051 ± 36 ） U/g Hb, RBC - MDA（3.73 ± 0.36） nmol/ml, Na ^＋ - K ^＋ ATPase leve（88.7 ± 20.1 ） μmolp/g Hb in control group with the diffetence being significant（ P 〈 0.05）. Conclusion： Align-aft transplantation of kidney tissu mass can ameliorate renal anemia caused by chronic renal failure and might be anew therapeutic means to treat CRF induced anemia.