多药耐药基因表达与急性髓细胞性白血病化疗效果的关系

1目的　探讨白血病细胞多药耐药 (MDR)基因表达与化疗效果的关系。 2方法　应用 MRK- 16和C1 2 9单克隆抗体对 2 0例急性髓细胞性白血病 (AML)进行了流式细胞术、细胞免疫化学、多聚酶链反应检查 ,并行细胞遗传学分析。 3结果　 18例初、复诊病人均有 MDR表达 ,但 MDR表达与完全缓解 (CR)率无线性关系 ,化疗未完全缓解者 CD 3 4细胞增多。 4结论　 MDR基因表达难估 AML 预后 ,CD 3 4细胞可作为 AML 预后的指标

CL INICAL REL EVANCE OF MDR-1/P170 EXPRESSION IN ACUTE MYEL OBL ASTIC LEUKEMIA

Objective To study the relationship between the expression of multidrug resistance gene in leukemic cells and the result of chm otherapy. Methods Expression of P170 encoded by the MDR-1gene were investigated by FACS(MRK- 16 ) ,RT-PCR ,imm unocytochemistry(C1 2 9) and cytogenetics respectvely in 2 0 patients with acute myeloblastic leukemia. Results 18out of2 0 clinical spcimens expressed MDR-1gene products.There was no correlation between MDR-1gene expression and CR.However,we found a highier level of CD 3 4positivity in those who failed in achievement of CR after chemotherapy. Conclusion The results implied that CD 3 4,not MDR gene expression was associated with a decreased CR rate.