葡萄糖消耗试验用于体外抗真菌药物敏感性试验

目的：评价葡萄糖消耗试验应用于体外抗真菌药物敏感性试验的可行性。方法：分析8种抗真菌药物与40株临床分离菌株在仅含葡萄糖为碳源的酵母氮培养基(YNBG)上孵育后葡萄糖的浓度变化，反映真菌细胞的受抑制程度，同时与NCCLS M27-A液基微量稀释法对比。结果：葡萄糖消耗试验在念珠菌和隐球菌的药敏测定中至少可以节省20小时，对须癣毛癣菌则至少可节省40小时；除两种方法测得的联苯苄唑MIC符合程度较低外，两种方法测得的两性霉素B、特比萘芬、伊曲康唑、酮康唑、咪康唑、氟康唑、环吡酮胺的MIC符合程度均较高。在评价由较低或较高浓度药物诱导的真菌细胞受抑制程度时，葡萄糖消耗试验更精确。结论：葡萄糖消耗试验具有客观量化、快捷、精确的优点，具有推广和应用价值。

Application of glucose consumption testing in vitro antifugal susceptibility test

Objective: To evaluate the feasibility of glucose consumption testing (GCT) in vitro antifungal susceptibility test. Methods: In vitro antifungal activities of 8 antifungal agents against 40 clinical isolates were determined both by measuring glucose consumption only containing carbon source in YNBG medium, and by NCCLS M27-A broth microdilution method. Results:Compared with routine tests, the modified method could save 20 hours for Candida albicans, 40 hours for T. mentagrophytes. For the 40 isolates, amphotericin B, fluconazole, itraconazole, terbinafine, miconazole, ketoconazole and ciclopirox olamine demonstrated high agreement in MICs between the two methods, whereas bifonazole produced less favorable MIC agreement. This method was able to provide precise MIC calculated with linear regression equation when MICs were beyond the range of concentrations tested. Conclusion :Glucose consumption testing may be an objective, quantitative, precise, quick and reliable tool for the in vitro determination of MICs of the 7 antifungal agents.