The effects of quercetin and isorhamnetin on oxidative modification of VLDL induced by Cu2+]

OBJECTIVE: To observe the effects of quercetin(Que) and isorhamnetin (Iso) on the oxidative modification of very low density lipoprotein (VLDL). METHODS: Human VLDL was prepared by one-step ultracentrifuge and oxidized by Cu2+ in vitro; Que and Iso in different concentrations were added to VLDL before incubation with Cu2+. The MDA, vitamin E contents and the activity of superoxide dismutase(SOD) in ox-VLDL were determined. RESULTS: Compared with ox-VLDL group, Que and Iso decreased MDA production in a dependent concentration-manner, Que and Iso also elevated Vit E level and SOD activity markedly. CONCLUSION: This experiment indicates that Que and Iso have similar inhibitory effects on the oxidative modification of VLDL.