首页 | 本学科首页   官方微博 | 高级检索  
     

骨髓单个核细胞和骨髓源内皮祖细胞移植促进血流重建的比较研究
引用本文:佟铸,谷涌泉,李建新,罗涛,张淑文,张建. 骨髓单个核细胞和骨髓源内皮祖细胞移植促进血流重建的比较研究[J]. 中国普通外科杂志, 2009, 18(12): 1259-1262
作者姓名:佟铸  谷涌泉  李建新  罗涛  张淑文  张建
作者单位:首都医科大学宣武医院,血管外科、首都医科大学血管外科研究所,北京,100053
基金项目:国家高技术研究发展计划(863) 基金资助项目(2006AA02A134);北京市自然科学基金资助项目(7072031);北京市委组织部优秀人才基金资助项目(20061D0501800265)。
摘    要:目的 比较骨髓单个核细胞(MNCs)和骨髓源内皮祖细胞(EPCs)移植促进血流重建的效果,探讨非内皮祖细胞在血流重建中的作用.方法 获取Lewis大鼠骨髓MNCs,部分MNCs在体外诱导分化为EPCs.采用Lewis大鼠建立单侧后肢缺血模型.建模后3 d,将模型鼠随机分为3组:(1)对照组(n=6),将0.8 mL D-Hank's液注入对照组大鼠缺血侧后肢;(2)MNC组(n=6),将8×10~6个骨髓MNC植入MNC组大鼠缺血侧后肢;(3)EPC组(n=6),将体外培养的8 × 10~6个EPC植入EPC组大鼠缺血侧后肢.细胞移植后3周行大鼠后肢动脉造影,检测缺血侧后肢侧支血管数;切取缺血侧后肢腓肠肌,分别行CD31和α-SMA免疫组化染色,计算毛细血管密度和小动脉密度.结果 MNC组毛细血管密度与EPC组差异无统计学意义[(31.67 ± 7.87)个/HP vs.(32.83±5.38)个/HP,P>0.05].而均高于对照组(19.67 ± 4.80个/HP)(P<0.05);MNC组侧支血管数与EPC组差异无统计学意义[(4.17±0.75)个vs.(4.50 4±1.38)个,P>0.05],但均高于对照组[(2.50 ± 1.5)个](P<0.05);MNC组小动脉密度与EPC组差异无统计学意义[(4.83 ± 1.47)个vs.(5.50 ± 2.35)个,P>0.05],亦均高于对照组[(2.17±0.98)个](P<0.05).结论 在骨髓干细胞移植治疗肢体缺血性疾病中,非内皮祖细胞在血流重建中所起的作用与EPC相似.

关 键 词:骨髓单个核细胞  内皮祖细胞  细胞移植  血流重建
收稿时间:2009-06-19
修稿时间:2009-09-20

Comparison of revascularization of bone marrow mononuclear cells and endothelial progenitor cells transplantation
TONG Zhu,GU Chong-Quan,LI Jian-Xin,LUO Chao,ZHANG Chu-Wen,ZHANG Jian. Comparison of revascularization of bone marrow mononuclear cells and endothelial progenitor cells transplantation[J]. Chinese Journal of General Surgery, 2009, 18(12): 1259-1262
Authors:TONG Zhu  GU Chong-Quan  LI Jian-Xin  LUO Chao  ZHANG Chu-Wen  ZHANG Jian
Affiliation:(Department of Vascular Surgery|Xuanwu Hospital|the Capital University of Medical Sciences, Beijing 100053, China)
Abstract:Objective:To compare the effect on revascularization of bone marrow mononuclear cells (BM/MNCs)and endothelial progenitor cells (EPCs) transplantation, and investigate  the roles of non-endothelial progenitor cells in angiogenesis.
Methods:BM/MNCs were harvested from Lewis rats bone marrow. The EPCs were obtained after BM/MNCs were cultured by EBM-2 medium for seven days. Lower limb ischemic model was made on Lewis rats。After limb ischemic model were made, 0.8ml D-Hanks, 8×106 BM/MNCs and 8×106 EPCs were injected into control group (n=6), BM/MNCs group (n=6) and EPCs group (n=6) respectively. At 3 weeks after cells transplantation, the quantity of collateral vascularization was observed with digital subtraction angiography(DSA). Meanwhile, CD31and α-SMA immunohistochemical stain were used to count the capillary and arteriolar density of the excised gastrocnemius muscle of the ischemic limb.
Results:There was no significant difference between BM/MNCs group and EPCs group in the density of the capillaries[(31.67±7.87)/HP vs(32.83±5.38)/HP,P>0.05],the number of collaterals [(4.17±0.75)vs(4.50±1.38),P>0.05)] and density of arterioles [(4.83±1.47)vs(5.50±2.35),P>0.05)], which were higher than those in control group, respectively.
Conclusions:Non-endothelial progenitor cells in angiogenesis has the same effects as EPC in the treatment of stem cells transplantation for ischemic disease of the limbs.
Keywords:Bone Marrow Mononuclear Cells  Endothelial Progenitor Cells  Cell Transplantation  Revascularization
本文献已被 万方数据 等数据库收录!
点击此处可从《中国普通外科杂志》浏览原始摘要信息
点击此处可从《中国普通外科杂志》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号