新疆维吾尔族妇女宫颈癌组织中HPV16型E6基因突变分析

背景与目的：高危型人乳头状瘤病毒16和18(human papillomavirus type16 and 18,HPV16,HPV18)是宫颈癌主要病因之一,尤其以HPV16最为常见,其中HPV16E6是主要癌基因之一。在一些地区,特定的E6基因突变株是宫颈癌发生的危险因素。新疆南部维吾尔族聚居区足宫颈癌高发区,我们已在前期的研究中发现该地区HPV16E6基因发生突变。本研究旨在检测该突变在新疆南部维吾尔族妇女宫颈癌组织中的分布规律,并探讨其与该地区宫颈癌高发的关系。方法：从35例中国新疆南部维吾尔族妇女宫颈癌活检标本中提取组织DNA作为模板,PCR扩增HPV16E6全长基因,PCR产物直接测序或克隆后测序,分析新疆维吾尔族妇女宫颈癌组织中HPV16E6基因的突变。结果：PCR检测结果表明宫颈癌组织中HPV16E6阳性率为82．86％(29／35);26例中E6分离片段的测序和序列分析表明,15例(57．69％)分离株E6基因与原型相同,另有11例(42．31％)E6基因突变,其中9例(34．62％)分离株发生了L83V突变,2例(7．69％)分离株发生rL83V／D63E突变。结论：中国新疆南部地区HPV16E6基囚发生变异,其原型和变异型在该地区维吾尔族宫颈癌患者巾的分布规律可能与该地区宫颈癌高发存在一定关系。

Analysis of human papillomavirus 16 E6 oncogene mutation in Xinjiang Uygur women with cervical carcinoma

BACKGROUND &OBJECTIVE: High-risk human papillomaviruses(HPVs),s uc h as HPV16, and HPV18,are major causes of cervical cancer (CC), and HPV16 was found most frequently in CC patients. HPV16E6 is one of major oncogenes. In some region, specific E6 mutation is considered as dangerous factor causing CC. Ther e is a very high incidence of CC in southern Xinjiang, where the Uygur are the m ajority. As we reported before, we found HPV16E6 mutation from this district. Th is study was designed to investigate distribution of the mutation in CC of Xinji ang Uygur women, and the relationship between the mutation and high incidence of CC in southern Xinjiang. METHODS: The tissue DNA was extracted from 35 CC biops ies of Xinjiang Uygur Women. HPV16E6 gene was amplified by polymerase chain reac tion (PCR) from the CC tissue DNA. The PCR fragments were sequenced and analyzed . RESULTS:The result of PCR showed that the positive rate of HPV16E6 was 82.86% (29/35); 26 of these 29 PCR fragments were sequenced and analyzed, 15 of them ma intained prototype (57.69%), 11 have L83V mutation (34.62%), and 2 have L83V/D 63E mutation (7.69%).CONCLUSIONS: There is mutation within the HPV16E6 gene in CC of Xinjiang Uygur women. Our research suggested that the distribution of HPV1 6 prototype and HPV16E6 mutation might be associated with high incidence of CC i n southern Xinjiang.