| A型H3579亚型流感多表位核酸疫苗小鼠实验免疫研究 |
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| 引用本文： | 南文龙,金宁一,鲁会军,陈义锋,赵翠青,田明尧,白靓,谭磊,张金双. A型H3579亚型流感多表位核酸疫苗小鼠实验免疫研究[J]. 免疫学杂志, 2009, 0(5) |
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| 作者姓名： | 南文龙 金宁一 鲁会军 陈义锋 赵翠青 田明尧 白靓 谭磊 张金双 |
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| 作者单位： | 军事医学科学院军事兽医研究所病毒室;吉林大学畜牧兽医学院;吉林大学药学院; |
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| 基金项目： | “863”重大项目(2006AA10A205);;科技支撑项目(2006BAD06A05) |
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| 摘 要： | 目的为了应对流感流行多亚型并存的局面,进行了多表位核酸疫苗设计,并观察其免疫小鼠的细胞和体液免疫应答反应。方法设计并合成了含有H3、H9的HA和H5N1NA中和表位,M、NP基因保守序列CTL表位的多表位基因盒(Epi)。采用pVAX载体对H5HA、H7HA及Epi进行融合表达。经肌肉注射免疫6～8周龄雌性Balb/c小鼠,ELISA法检测小鼠血清中的针对H3579亚型流感抗体。三免后2周,分离脾淋巴细胞,进行T淋巴细胞转化试验和T淋巴细胞亚类数量检测。结果免疫小鼠获得了针对H3579亚型流感的体液和细胞免疫反应。结论完整HA抗原结合多表位的DNA疫苗模式的成功,预示了该DNA疫苗可能可以有效应对当前多种亚型流感并存的局面,并为其他种类流感疫苗的研究奠定了基础。
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| 关 键 词： | 流感 核酸疫苗 多表位 |
Construction and immunization research of muti-epitopes DNA vaccine for anti-multi-serotypes (H3,5,7,9) of influenza A virus |
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| NAN Wenlong,JIN Ningyi,LU Huijun,CHEN Yifeng,ZHAO Cuiqing,TIAN Mingyao,BAI Liang,TAN Lei,ZHANG Jingshuang Institute of Military Veterinary,Academy of Military Medical sciences,Changchun ,China. Construction and immunization research of muti-epitopes DNA vaccine for anti-multi-serotypes (H3,5,7,9) of influenza A virus[J]. Immunological Journal, 2009, 0(5) |
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| Authors: | NAN Wenlong JIN Ningyi LU Huijun CHEN Yifeng ZHAO Cuiqing TIAN Mingyao BAI Liang TAN Lei ZHANG Jingshuang Institute of Military Veterinary Academy of Military Medical sciences Changchun China |
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| Affiliation: | NAN Wenlong,JIN Ningyi,LU Huijun,CHEN Yifeng,ZHAO Cuiqing,TIAN Mingyao,BAI Liang,TAN Lei,ZHANG Jingshuang Institute of Military Veterinary,Academy of Military Medical sciences,Changchun 130062,China |
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| Abstract: | Objective To design a muti-epitopes DNA vaccine and evaluate its humoral and cellular immune responses in mouse model.Methods A multi-epitopes box(Epi)was constructed,which contained neutralizing epitopes of H3/H9 HA and H5N1 NA genes,as well as conserved CTL epitopes of M and NP genes.The co-expressing plasmid(pVAX1-H7HA-Epi-H5HA)containing H5HA,H7HA,and Epi was constructed using pVAX1 expression vector as the backbone.Six-week-old female Balb/c(H-2d)mice were immunized three times in a two weeks interval ... |
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| Keywords: | influenza DNA vaccine multi-epitopes |
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