免疫磁珠技术在结直肠癌腹腔内脱落癌细胞检测中的应用

目的 探讨免疫磁珠(IMB)检测方法用于结直肠癌腹腔内脱落癌细胞的检测价值.方法 收集80例结直肠癌患者术中的腹腔冲洗液,分别进行IMB及常规腹腔冲洗液细胞学检查(PLC),检测腹腔内脱落癌细胞,并以10例妇产科子宫肌瘤手术患者腹腔冲洗液标本作为对照组.结果 PLC检测阳性率为8.8％(7/80),IMB检测阳性率为28.8％(23/80),PLC检测阳性的病例,IMB均为阳性.IMB检出率明显高于PLC(x2=10.503,P=0.001).对照组两种方法检测均为阴性结果.结论 IMB对于结直肠癌腹腔脱落癌细胞检出率较PLC有较大提高,为腹腔癌细胞微转移的发现提供了一个切实可行的有效途径.

Isolation of intraperitoneal free cancer cells from colorectal cancer by immunomagnetic beads

Objective To explore the value of immuomagnetic beads（IMB） technique for detection of intraperitoneal free cancer cells from colorectal cancer. Methods Peritoneal lavage fluid was obtained from 80 patients with colorectal cancer during laparotomy. Peritoneal lavage cytology （PLC） and IMB were used to detect free cancer cells in peritoneal lavage fluid. 10 patients with hysteromyoma during laparotomy were enrolled into the control group. Results The positive rate of PLC was 8.8% （7/80）, the positive rate of IMB was 28.8% （23/80）. The positive case after useing PLC detect, IMB detect also was positive. The detected samples of control group were negative by these two methods. IMB was superior to PLC （X2 = 10. 503, P = 0. 001 ）. Conclusion IMB was more sensitive and specific than PLC,which could provide a effective method for finding intraperitoneal free cancer cells.