结缔组织生长因子在大鼠肺纤维化模型中的蛋白表达

目的 :了解结缔组织生长因子 (connectivetissuegrowthfactor ,CT GF)在大鼠肺纤维化模型中的表达状况及其与肺纤维化之间的关系。方法 :42只Wistar大鼠随机分为二组。模型组 (M组 ) :气管内灌注博莱霉素 ,对照组 (C组 ) :气管内灌注生理盐水。于灌注后第 7、14、2 8天每组分别处死大鼠 7只。制备大鼠肺组织切片。用免疫组化SABC法分别检测二组大鼠肺组织中CTGF及转化生长因子 β1 (TGF β1 )、纤维连接蛋白 (FN )在肺内的表达水平。结果 :M组CTGF及TGF β1 、FN在肺内的表达水平在各时间点均显著高于C组 (P <0 .0 5 )。结论 :CTGF作为TGFβ1 的下游因子 ,可能通过促进细胞外基质(ECM)如FN和胶原蛋白等合成而在博莱霉素诱导的肺纤维化形成过程中起重要作用

Express of Connective Tissue Growth Factor Protein in Lungs of Rats with Pulmonary Fibrosis

Objective: To study the role of connective tissue growth factor(CTGF) in rats with pulmonary fibrosis by bleomycin induced.Method: Fourty-two wistar rats were divided into two groups.model group treated with bleomycin intratracheally(GroupM),and the normal control group treated with N.S(GroupC).Seven rats were killed on the 7th,14th,28th after bleomycin treatment respectively. After histological process,The expression of CTGF、TGF-β 1、Fn protein were examined by using SABC immunohistochemical method.Results: The levels of the expression of CTGF、 TGF-β 1、Fn protein was increased significantly in the Group M as compared with the Group C(P<0.05).Conclusion: CTGF,downstream cytokine of TGF-β 1,may play an important role in the development of the pulmonary fibrosis through promoting the synthesis of ECM.