柿叶黄酮对糖尿病小鼠肝脏的保护作用

目的探讨柿叶黄酮对四氧嘧啶糖尿病小鼠肝脏的保护作用。方法尾静脉注射四氧嘧啶（60mg／kg）制备糖尿病模型，分为柿叶黄酮0．5、2．5、5 g/kg组和模型组，另设正常对照组，连续灌胃治疗2周。于末次给药3h后，摘眼球取血，分离血清，测定ALT和AST活性；取肝脏称重，计算肝脏指数；4％中性甲醛固定肝脏，石蜡切片，H．E染色，显微镜观察。结果糖尿病小鼠肝脏指数明显增加；血清ALT和AST提高；肝细胞失去正常小叶结构，肝细胞发生空泡样变性，淋巴细胞浸润明显。经柿叶黄酮治疗后，小鼠的肝脏指数和血清中AST及ALT水平降低；肝细胞的索状结构较清晰，肝细胞损伤减轻，淋巴细胞浸润减少，其中，柿叶黄酮5g／kg组治疗效果最佳。结论柿叶黄酮能抑制四氧嘧啶糖尿病小鼠的肝肿大，保护肝功能，减轻肝脏组织病理变化，有效的延缓糖尿病引起的肝脏损伤。

Protective effect of .Persimmon leaf flavonoids on the liver in diabetic mice

Objective To investigate the protective effect of. Persimmon leaf flavonoids （PLF） on the liver in alloxan-induced diabetic mice. Methods The diabetic mice model was established by tail vein injection of alloxan （60 mg/kg）. The diabetic mice were divided into four groups treated with PLF0. 5,2. 5,5 g/ kgand model control,while a normal conrol group was set up simultaneously. The drugs were given ig for two weeks. Three hours after the last treatment, the blood was taken by exposing the eye ball. separated from the serum, determination of the activity of ALT and AST; liver weight, liver index calculation ;4% neutral formalin- fixed liver. The hepatic tissue was embedded with paraffin, sectioned, H-E staining, the histopathology was observed with microscope. Results Diabetic mice significantly increased the liver index;The activity of serum ALT and AST increased;The loss of normal hepatic lobular structure of cells, liver cells vacuolated degeneration and lymphocytic infiltration significantly. After treatment by PLF in diabetic mice, liver index and serum levels of AST and ALT decreased. Hepatocyte cord-like structure more clear, to reduce liver cell injury, reduced lymphocyte infiltration, included PLF 200 mg kg-1 best treatment Conclusion PLF can inhibit alloxan-induced diabetic mice hepatomegaly, the protection of liver function, reduce the pathological changes in liver tissue, and delay the progression of injury of liver induced by diabetes mellitus.