RNA干扰c-Met基因表达对非小细胞肺癌侵袭和迁移及顺铂敏感性的影响

[目的]研究c-Met基因干扰后对肺癌细胞株95D侵袭、迁移能力和化疗药物敏感性的影响。[方法]分别采用免疫组化SP法和WesternBlot技术检测肺癌组织及不同肺癌细胞株中Met蛋白的表达情况。将c-MetshRNA质粒转染人肺癌细胞株95D．通过WestemBlot检测转染效率；Transwell小室和划痕愈合实验测定细胞体外侵袭和迁移能力：四甲基偶氮唑法（Mar法）检测细胞的增殖情况及顺铂敏感性。[结果]Met蛋白在NSCLC组织中的表达明显高于癌旁正常组织，同时高侵袭性的肺癌细胞株（95D、801D）中Met蛋白的表达也较高。c-Met基因干扰能明显降低95D细胞的侵袭和迁移能力，并显著提高其对顺铂的敏感性。[结论]c-Met基因可作为一个重要的靶点应用于非小细胞肺癌治疗。

The Effects of RNA Interference to c-Met Gene on Invasion and Migration of Human Non-small Cell Lung Cancer and the Sensitivity to Cisplatin

[Purpose] To investigate the effects of c-Met gene interference on invasion and migration ability and chemotherapeutic drugs sensitivity in lung cancer 95D cell line. [Methods] c-MET protein expression was detected in lung cancer tissues and different lung cancer cell lines using immunohistochemistry and Western Blot technology, c-Met shRNA plasmid was transfected into lung cancer 95D cells,then the ability of cell invasion and migration was detected by transwell chambers in vitro invasion assay and wound-healing assay. Cell proliferation and the sensitivity of lung cancer cell to cisplatin were determined by MTY assay. [Results] Met protein expression in NSCLC tissues was significantly higher than that in cancer adjacent tissues. Meanwhile,Met protein expression in invasive lung cancer cell line （95D, 801D） was higher than that in low invasive lung cancer cell line （95C） and in BEAS-2B cell. In addition, c-Met gene silence significantly inhibited the migration and invasion ability of 95D cells and improved its sensitivity to cisplatin.[Conclusion] c-Met gene may have the potential as a thera- peutic target using in the treatment for human non-small cell lung cancer.