脑血通片对脑缺血损伤的保护作用

目的 观察脑血通片对脑缺血损伤的保护作用并探讨其作用机制.方法 采用小鼠全脑缺血、小鼠双侧颈总动脉及迷走神经结扎、小鼠全脑缺血再灌注、大鼠急性不完全脑缺血及胶原蛋白-肾上腺素混合诱导剂诱导的小鼠肺血栓实验模型,观察脑血通低剂量组(NXT-L组)与脑血通高剂量组(NXT-H组),对脑缺血损伤的保护作用.结果 NXT-H延长小鼠断头喘息的存活时间为(23.26±1.76)s;NXT-L与NXT-H延长小鼠双侧颈总动脉及迷走神经结扎后的死亡时间分别为:(128.33±3.11)s、(1316±7.67)s;NXT-H可以升高小鼠全脑缺血再灌注模型SOD值为:(150.02±18.51)U/mgprot;NXT-L与NXT-H降低小鼠全脑缺血再灌注模型MDA值分别为:(26.80±1.92)nmol/mgprot、(26.43±1.90)nmol/mgprot;NXT-L与NXT-H降低急性不完全脑缺血模型MDA值分别为:(6.36±1.67)nmol/mgprot、(5.78±0.90)nmol/mgprot;NXT-H降低脑指数为:(0.71±0.04)%;NXT-H降低胶原蛋白-肾上腺素混合诱导剂诱导的小鼠肺血栓实验模型中小鼠死亡数+偏瘫未恢复数为:(2+9=11)只.结论 NXT可以通过升高SOD活性,降低MDA的生成,降低脑血管通透性,发挥对脑组织的保护作用;可以降低胶原蛋白-肾上腺素混合诱导剂诱导的小鼠肺血栓实验模型中小鼠死亡数+偏瘫未恢复数.

Experimental study on the protective effects of Naoxuetong tablets on cerebral ischemia in mice

Objective To explore the protective effects and possible mechanism of Naoxuetong tablets on cerebral ischemia in mice.Methods The animal models were established including global brain ischemia models in mice, bilateral common carotid artery ligation and vagas nerve ligation models in mice,global brain ischemia-reperfusion (I/R) in mice, pulmonary embolism models in mice induced by collagen-epinephrine combination,and acute incomplete brain ischemia models in rats,then the protective effects of Naoxuetong low-dose group (NXT-L group) and Naoxuetong high-dose group (NXT-H group) on cerebral ischemia injury were observed.Results The lengthening survival time of decapitation gasping in mice of NXT-H group was (23.26±1.76)s,and the lengthening survival time after bilateral common carotid artery ligation and vagas nerve ligation in NXT-Lgroup and NXT-H group was (128.33±3.11)s,(1316±7.67)s,respectively.The levels of SOD were (150.02±18.51)U/mgprot in mice with global brain ischemia-reperfusion,which were increased by NXT-H,moreover, NXT-L and NXT-H decreased the MDA levels in mice with global brain ischemia-reperfusion by (26.80±1.92)nmol/mgprot,(26.43±1.90)nmol/mgprot,respectively.However NXT-L and NXT-H decreased the MDA levels in rats with incomplete brain ischemia-reperfusion by (6.36±1.67)nmol/mgprot,(5.78±0.90)nmol/mgprot,respectively.NXT-H could decrease cerebral index by (0.71±0.04)%,and the death number of mice+hemiplegia number (non-recovery) decreased by NXT-H were 11 mice (2+9) in mice models with pulmonary embolism induced by collagen-epinephrine combination.Conclusion NXT can produce protective effects on brain tissue of mice by increasing the activity of SOD, decreasing the production of MDA and reducing cerebral vascular permeability, moreover, which can decrease the death number of mice+hemiplegia number (non-recovery) in mice models with pulmonary embolism induced by collagen-epinephrine combination.