胆囊结石患者胆汁、粘膜和结石需氧菌和厌氧菌脱氧核糖核酸研究

目的研究胆囊结石患者胆汁、粘膜和结石细菌脱氧核糖核酸(DNA),提供细菌在胆囊结石形成中的分子生物学汪据.方法采用聚合酶链式反应(PCR)对70例胆汁、粘膜和结石标本中5种需氧菌包括大肠埃氏杆菌、克雷伯杆菌、幽门螺杆菌、变形杆菌、伤寒杆菌和4种厌氧菌包括脆弱类杆菌、消化球菌、产气荚膜杆菌、梭形芽胞杆菌DNA进行检测.结果胆汁、粘膜、结石和门静脉血中需氧菌和厌氧菌DNA存在率分别为80%,86%,61%,36%和33%,50%,24%,46%;两类细菌DNA共同存在分别为29%,44%,16%,14%.结论胆汁、粘膜、结石中存在多细菌DNA,胆结石核心形成可能与细菌感染有关.

A study on DNA of aerobic and anaerobic bacteria in bile, mucosa and stone in gallstone patients

AIM To study bacteria deoxyribonucleic acid (DNA) in bile, mucus and stone, and provide evidence of molecular biology of bacteria in gallstones formation. METHODS DNA of five aerobic bacteria including Bacillus Coli, Kelbsiella Pneumonie, Bacillus Typhoid, Helicobacter pylori, Bacillus Proteus and four anaerobic bacteria including Bacteroides Fragilis, Peptococcus, Clostridium and Bacillus Perfringens in previous samples was tested with polymerase chain reaction (PCR) in 70 gallstone patients.RESULTS DNA of aerobic and anaerobic bacteria in bile, mucus stone, portal vein blood was 80%, 86%, 61% and 36% and 33%, 50%, 24% and 46% respectively; DNA of aerobic and anaerobic bacteria coexistence was 29%, 44%, 16% and 14% respectively. CONCLUSION There are multiple bacteria in the bile, mucus and stone. Gallstone nuclear formation may be related to bacteria infection.