nm23H1蛋白在喉癌中的表达及临床意义

为探讨喉癌ｎｍ ２３－Ｈ１ 蛋白表达与其生物学行为及淋巴结转移的关系，作者采用ＬＳＡＢ免疫组化方法，研究了８６ 例喉癌手术标本中ｎｍ ２３－Ｈ１ 蛋白的表达情况。结果：喉癌原发灶中ｎｍ ２３－Ｈ１ 蛋白表达的阳性率为６６．３％ ，其中无区域淋巴结转移者阳性率为９０．５％ ，伴区域淋巴结转移者阳性率为４３．２％ ，两者比较差异显著（Ｐ＜ ０．００５）。随着喉癌浸润深度和临床分期的进展，原发灶中ｎｍ ２３－Ｈ１ 蛋白表达阳性率逐渐下降；随着癌细胞组织学分级的升高，ｎｍ ２３－Ｈ１ 蛋白表达阳性率逐渐增高。由此提示，ｎｍ ２３－Ｈ１ 基因在喉癌的浸润和淋巴结转移过程中发挥了负性调控作用。

nm23-H1 Protein Expression and Its Clinical Significance in Human Laryngeal Carcinoma

The Objective of the study on the expression of nm23 H1 protein in human laryngeal carcinoma, was to explore the role of nm23 gene in lymph node metastasis and invasion. The expression of nm23 H1 protein was immunohistochemically examined in 86 resected human laryngeal carcinomas. Anti nm23 H1 monoclonal antibody was used. The results showed that 66.3%(57/86) of the cases were positive for nm23 H1 protein expression in the primary lesions. The positive staining rate (90.5%,38/42) of the patients without regional lymph node metastasis, was significantly higher than that of patients with nodal involvement (43.2%,19/44).Inverse relationship were found between the nm23 H1 protein expression in the primary lesions and the depth of tumor invasion or the advance of clinical stage. Expression of nm23 H1 protein was positively associated with the ascending histological grade of the lesion, and the relationship was significant. The above data suggest that nm23 gene plays an importnat role in the control of lymph node metastasis and invasion in laryngeal carcinomas.